Abstract
Antibodies are versatile biological molecules with widespread applications in research and medicine. This protocol outlines the generation of monoclonal IgG antibodies from Chinese hamster ovary cells. It includes steps for cell maintenance, transient transfection, and antibody purification via protein A affinity chromatography. The methods described are intended for the production of milligram amounts of protein but can be adapted for most small- to mid-scale applications.
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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Stuart, L. (2024). Production and Purification of Antibodies in Chinese Hamster Ovary Cells. In: Bradfute, S.B. (eds) Recombinant Glycoproteins. Methods in Molecular Biology, vol 2762. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3666-4_11
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DOI: https://doi.org/10.1007/978-1-0716-3666-4_11
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3665-7
Online ISBN: 978-1-0716-3666-4
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