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Targeted Analysis of Permethylated N-Glycans Using MRM/PRM Approaches

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Recombinant Glycoproteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2762))

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Abstract

Targeted mass spectrometric analysis is widely employed across various omics fields as a validation strategy due to its high sensitivity and accuracy. The approach has been successfully employed for the structural analysis of proteins, glycans, lipids, and metabolites. Multiple reaction monitoring (MRM) and parallel reaction monitoring (PRM) have been the methods of choice for targeted structural studies of biomolecules. These target analyses simplify the analytical workflow, reduce background interference, and increase selectivity/specificity, allowing for a reliable quantification of permethylated N-glycans in complex biological matrices.

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Acknowledgments

This work was supported by grants from the National Institutes of Health, NIH (1R01GM112490-04, 1R01GM130091-01). The work is also supported by grants from Robert A. Welch Foundation (No. D-0005) and The CH Foundation.

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Author notes

  1. Cristian D. Gutierrez Reyes and Akeem Sanni contributed equally with all other contributors.

Authors and Affiliations

  1. Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA

    Cristian D. Gutierrez Reyes, Akeem Sanni, Moyinoluwa Adeniyi, Parisa Ahmadi, Mojgan Atashi, Sherifdeen Onigbinde & Yehia Mechref

  2. Department of Food Biochemistry, Faculty of Food Science, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland

    Damir Mogut

Authors
  1. Cristian D. Gutierrez Reyes
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  2. Akeem Sanni
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  3. Damir Mogut
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  4. Moyinoluwa Adeniyi
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  5. Parisa Ahmadi
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  6. Mojgan Atashi
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  7. Sherifdeen Onigbinde
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  8. Yehia Mechref
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Corresponding author

Correspondence to Yehia Mechref .

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Editors and Affiliations

  1. Center for Global Health, University of New Mexico Health Sciences Center, Albuquerque, NM, USA

    Steven B. Bradfute

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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Gutierrez Reyes, C.D. et al. (2024). Targeted Analysis of Permethylated N-Glycans Using MRM/PRM Approaches. In: Bradfute, S.B. (eds) Recombinant Glycoproteins. Methods in Molecular Biology, vol 2762. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3666-4_15

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  • DOI: https://doi.org/10.1007/978-1-0716-3666-4_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3665-7

  • Online ISBN: 978-1-0716-3666-4

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