Abstract
Uroteuthis edulis is a widespread neritic cephalopod with a complex population structure and wide migration range. The life history of this species is still ambiguous. Statolith microchemistry is an effective indicator to reveal individual life history and migration route. In this study, the statolith microchemistry of U. edulis in different stocks was determined by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS), and the statolith diameter was clustered by using a multiple regression tree model (MRT) to analyze differences in the ratio of various elements to Ca at different life history stages. The analysis results showed that sodium (Na), magnesium (Mg), iron (Fe), strontium (Sr), and Barium (Ba) were the effective elements in four stocks. Five clusters were divided according to MRT and represented five ontogenetic stages. The Sr/Ca, Ba/Ca, and Mg/Ca ratios in summer and autumn stocks were significantly different from those of spring and winter stocks (P < 0.05). From the embryonic stage to sub-adult stage, the Sr/Ca ratio in spring and winter stocks was higher than those of summer and autumn stocks. The Sr/Ca ratio in spring, summer, and winter stocks were significantly different among different ontogenetic stages (P < 0.05). The Ba/Ca ratio in the four stocks were significantly different between larva and adults (P < 0.05). Therefore, the Sr/Ca and Ba/Ca ratios can be selected as the key indicators to speculate on the migration route of U. edulis. This study improves understanding of the statolith microchemistry differences among different stocks of U. edulis.
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Acknowledgements
The authors acknowledge the financial support from National Science Foundation of China (42306117), and Fund of Key Laboratory of Open-Sea Fishery Development, Ministry of Agriculture, P. R. China (LOF 2018-02). Moreover, the authors acknowledge the reviewers for their comments.
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Squid samples were randomly selected from fisheries catch taken by Chinese trawler fleets. Thus, the samples are part of the fisheries catch (with specimens being already dead when sampled). The samples were taken with permission from Shanghai Ocean University, which is in charge of oceanic fisheries research in China (Hu et al. 2021). The sampling protocol had been evaluated and approved by Shanghai Ocean University. The samples were taken strictly following the protocol. The sampled squid catch (dead squid) were frozen and then dissected in the laboratory. We confirm that the fisheries from which the squid were obtained were conducted by a legitimate Chinese company with appropriate permits to fish in the waters described in the paper. We also confirm that none of the squid was collected for the purpose of this study. They were caught as fisheries catch.
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Li, N., Han, P., Chen, X. et al. Variation of statolith microchemistry among stocks of Uroteuthis edulis in the East China Sea. Fish Sci 89, 747–759 (2023). https://doi.org/10.1007/s12562-023-01714-5
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DOI: https://doi.org/10.1007/s12562-023-01714-5