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Fish cell line: depositories, web resources and future applications

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Abstract

Cell lines are important bioresources to study the key biological processes in the areas like virology, pathology, immunology, toxicology, biotechnology, endocrinology and developmental biology. Cell lines developed from fish organs are utilized as a model in vitro system in disease surveillance programs, pharmacology, drug screening and resolving cases of metabolic abnormalities. During last decade, there were consistent efforts made globally to develop new fish cell lines from different organs like brain, eye muscles, fin, gill, heart, kidney, liver, skin, spleen, swim bladder, testes, vertebra etc. This increased use and development of cell lines necessitated the establishment of cell line depositories to store/preserve them and assure their availability to the researchers. These depositories are a source of authenticated and characterized cell lines with set protocols for material transfer agreements, maintenance and ship** as well as logistics enabling cellular research. Hence, it is important to cryopreserve and maintain cell lines in depositories and make them available to the research community. The present article reviews the current status of the fish cell lines available in different depositories across the world, along with the prominent role of cell lines in conservation of life on land or below water.

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Acknowledgements

The authors are grateful to the Secretary, DARE and Director General, ICAR, Ministry of Agriculture and Farmers’ Welfare, New Delhi; Dr. J K Jena, DDG (Fy.), ICAR, New Delhi, India; and Director, ICAR-NBFGR, Lucknow, for their support, encouragement and guidance. The authors are also thankful to the Department of Biotechnology, Ministry of Science and Technology, Government of India, New Delhi, for the financial support to execute the National Fish Cell Line Repository (Phase I and II) in project modes at ICAR-NBFGR, Lucknow, India.

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  1. ICAR-National Bureau of Fish Genetic Resources, Lucknow, Uttar Pradesh, 226 002, India

    Murali S. Kumar, Vijay Kumar Singh, Akhilesh Kumar Mishra, Basdeo Kushwaha, Ravindra Kumar & Kuldeep Kumar Lal

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  1. Murali S. Kumar
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  2. Vijay Kumar Singh
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MSK: Draft manuscript preparation, VKS: Literatures collection and table compilation, AKM: Compilation of information of repository databases and reference collection/ compilation, BK: Editing draft manuscript, RK: Information mining from various depository databases, draft manuscript editing and improvement, KKL: Guidance and suggestions/improvement in manuscript.

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Correspondence to Ravindra Kumar.

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Kumar, M.S., Singh, V.K., Mishra, A.K. et al. Fish cell line: depositories, web resources and future applications. Cytotechnology 76, 1–25 (2024). https://doi.org/10.1007/s10616-023-00601-2

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