Abstract
For some highly endangered species there are too few reproductively capable animals to maintain adequate genetic diversity, and extraordinary measures are necessary to prevent their extinction. Cellular reprogramming is a means to capture the genomes of individual animals as induced pluripotent stem cells (iPSCs), which may eventually facilitate reintroduction of genetic material into breeding populations. Here, we describe a method for generating iPSCs from fibroblasts of mammalian endangered species.
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Acknowledgment
This work was supported by the Esther O’Keefe Foundation and the Millipore Foundation. S.M. was supported by a Bridges to Stem Cell Research grant from the California Institute for Regenerative Medicine (TB1-01186).
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Ben-Nun, I.F., Montague, S.C., Houck, M.L., Ryder, O., Loring, J.F. (2015). Generation of Induced Pluripotent Stem Cells from Mammalian Endangered Species. In: Verma, P., Sumer, H. (eds) Cell Reprogramming. Methods in Molecular Biology, vol 1330. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2848-4_10
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DOI: https://doi.org/10.1007/978-1-4939-2848-4_10
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2847-7
Online ISBN: 978-1-4939-2848-4
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