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Forster Resonance Energy Transfer (FRET) to Visualize Protein–Protein Interactions in the Plant Cell
Forster resonance energy transfer (FRET) is an efficient method to visualize the protein–protein interaction in living cells. This technique is based... -
Measuring Protein–Protein Interactions in Cells using Nanoluciferase Bioluminescence Resonance Energy Transfer (NanoBRET) Assay
Protein–protein interactions (PPIsProtein-protein interactions (PPIs)) are increasingly recognized for their roles in functional cellular networks... -
Cross-protein transfer learning substantially improves disease variant prediction
BackgroundGenetic variation in the human genome is a major determinant of individual disease risk, but the vast majority of missense variants have...
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KT-AMP: Enhancing Antimicrobial Peptide Functions Prediction Through Knowledge Transfer on Protein Language Model
As the overuse of antibiotics enhances the resistance of various microbial pathogens to antibiotics, antimicrobial peptides (AMPs) have the potential... -
Protein–Protein Interactions: Co-immunoprecipitation
Proteins often do not function as single substances but rather as team players in a dynamic network. Growing evidences show that protein–protein... -
Lipid Transfer Protein Genes Are Differently Expressed in Rat Soleus Muscle at Early and Later Periods of Muscle Disuse
AbstractThis work reports analysis of lipid transfer protein (LTPs) mRNA levels in rat soleus muscle after 12-h and 14-day hindlimb suspension (HS)....
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Resonance Energy Transfer
One way that an excited molecule can return to the ground state is to transfer the excitation energy to another molecule. This process, resonance... -
Studying Protein–Protein Interactions at Plasmodesmata by Measuring Förster Resonance Energy Transfer
Plasmodesmata (PD) provide interconnectivity between plant cells to enable the intercellular transport and communication that is requisite to... -
Measuring Protein-Protein Interactions of Melatonin Receptors by Bioluminescence Resonance Energy Transfer (BRET)
The melatonin receptor subfamily belongs to the G protein-coupled receptor superfamily and consists of three members in mammals, MT1, MT2, and GPR50.... -
The revealing of a novel lipid transfer protein lineage in green algae
BackgroundNon-specific lipid transfer proteins (nsLTPs) are a group of small and basic proteins that can bind and transfer various lipid molecules to...
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Protein–Protein Interaction: Bacterial Two Hybrid
The bacterial two-hybridBacterial two-hybrid (BACTH, for “Bacterial Adenylate Cyclase-based Two-Hybrid”) system is a simple and fast genetic approach... -
A multitask transfer learning framework for the prediction of virus-human protein–protein interactions
BackgroundViral infections are causing significant morbidity and mortality worldwide. Understanding the interaction patterns between a particular...
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Protein–Protein Interaction: Tandem Affinity Purification in Bacteria
The discovery of protein–protein interaction networks can lead to the unveiling of protein complex(es) forming cellular machinerie(s) or reveal... -
Protein–Protein Interactions: Oxidative Bacterial Two Hybrid
Protein–protein interaction studies are essential to understand how proteins organize themselves into interaction networks and thus influence... -
Gene Transfer
In addition to vertical gene transfer between generations, microbes often transfer their genes horizontally. Horizontal gene transfer contributes... -
Protein-Protein Binding Kinetics by Biolayer Interferometry
The specific kinetics and thermodynamics of protein-protein interactions underlie the molecular mechanisms of cellular functions; hence the... -
ANKFY1 bridges ATG2A-mediated lipid transfer from endosomes to phagophores
Macroautophagy is a process that cells engulf cytosolic materials by autophagosomes and deliver them to lysosomes for degradation. The biogenesis of...
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Protein–Protein Interactions: Yeast Two Hybrid
The yeast two-hybridYeast two-hybrid (Y2H) systemTwo-hybrid system is a powerful and commonly used genetic tool to investigate the interaction... -
HMGB1 promotes mitochondrial transfer between hepatocellular carcinoma cells through RHOT1 and RAC1 under hypoxia
Mitochondrial transfer plays an important role in various diseases, and many mitochondrial biological functions can be regulated by HMGB1. To explore...
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Protein language models can capture protein quaternary state
BackgroundDetermining a protein’s quaternary state, i.e. the number of monomers in a functional unit, is a critical step in protein...