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Protein-Protein Binding Kinetics by Biolayer Interferometry

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Advanced Technologies for Protein Complex Production and Characterization

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1453))

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Abstract

The specific kinetics and thermodynamics of protein-protein interactions underlie the molecular mechanisms of cellular functions; hence the characterization of these interaction parameters is central to the quantitative understanding of physiological and pathological processes. Many methods have been developed to study protein-protein interactions, which differ in various features including the interaction detection principle, the sensitivity, whether the method operates in vivo, in vitro, or in silico, the temperature control, the use of labels, immobilization, the amount of sample required, the number of measurements that can be accomplished simultaneously, or the cost. Bio-Layer Interferometry (BLI) is a label-free biophysical method to measure the kinetics of protein-protein interactions. Label-free interaction assays are a broad family of methods that do not require protein modifications (other than immobilization) or labels such as fusions with fluorescent proteins or transactivating domains or chemical modifications like biotinylation or reaction with radionuclides. Besides BLI, other label-free techniques that are widely used for determining protein-protein interactions include surface plasmon resonance (SPR), thermophoresis, and isothermal titration calorimetry (ITC), among others.

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Acknowledgments

This work was funded by the Spanish Ministerio de Ciencia, Innovación y Universidades-FEDER grants RTI2018-102242-BI00 (MCV), the Spanish Ministerio de Ciencia e Innovación-Recovery, Transformation and Resilience Plan (PRTR) grant PDC2022-133713-I00 (MCV), grant S2022/BMD-7278 of the Regional Government of Madrid (MCV), the European Commission – NextGenerationEU through CSIC’s Global Health Platform (“PTI Salud Global”) (SGL2103020) (MCV), and the CSIC Special Intramural Grant PIE201620E064 (MCV). It was additionally supported by the Research Network on Complement in Health and Disease (RED2022-134750-T). KdlP was supported by an Industrial PhD grant (IND2018-010094) awarded by the Spanish Ministerio de Economía y Competitividad. JSL acknowledges the support of the PhD program in Molecular Biosciences of the Universidad Autónoma de Madrid (UAM) and the Ministry of Education, Culture and Sports of Spain (FPU Grant 17/06090). KdlP acknowledges the support of the PhD program in Biochemistry, Molecular Biology and Biomedicine of the Universidad Complutense de Madrid (UCM).

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Authors and Affiliations

  1. Centro de Investigaciones Biológicas Margarita Salas (CIB-CSIC), Madrid, Spain

    Jorge Santos-López, Sara Gómez & M. Cristina Vega

  2. Universidad Europea de Madrid, Madrid, Spain

    Sara Gómez

  3. Abvance Biotech SL, Madrid, Spain

    Francisco J. Fernández

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  1. Jorge Santos-López
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  2. Sara Gómez
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Correspondence to M. Cristina Vega .

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  1. Centro de Investigaciones Biologicas Margarita Salas (CIB-CSIC), Madrid, Spain

    M. Cristina Vega

  2. Abvance Biotech SL, Madrid, Spain

    Francisco J. Fernández

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Santos-López, J., Gómez, S., Fernández, F.J., Vega, M.C. (2024). Protein-Protein Binding Kinetics by Biolayer Interferometry. In: Vega, M.C., Fernández, F.J. (eds) Advanced Technologies for Protein Complex Production and Characterization. Advances in Experimental Medicine and Biology, vol 1453. Springer, Cham. https://doi.org/10.1007/978-3-031-52193-5_6

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