Search
Search Results
-
How autoreactive thymocytes differentiate into regulatory versus effector CD4+ T cells after avoiding clonal deletion
Thymocytes bearing autoreactive T cell receptors (TCRs) are agonist-signaled by TCR/co-stimulatory molecules to either undergo clonal deletion or to...
-
Direct presentation of inflammation-associated self-antigens by thymic innate-like T cells induces elimination of autoreactive CD8+ thymocytes
Upregulation of diverse self-antigens that constitute components of the inflammatory response overlaps spatially and temporally with the emergence of...
-
Development of double-positive thymocytes at single-cell resolution
BackgroundT cells generated from thymopoiesis are essential for the immune system, and recent single-cell studies have contributed to our...
-
Liver X receptor β is required for the survival of single-positive thymocytes by regulating IL-7Rα expression
Liver X receptors (LXRs) are known as key transcription factors in lipid metabolism and have been reported to play an important role in T-cell...
-
Mannosylated glycans impair normal T-cell development by reprogramming commitment and repertoire diversity
T-cell development ensures the formation of diverse repertoires of T-cell receptors (TCRs) that recognize a variety of antigens. Glycosylation is a...
-
Oleic acid availability impacts thymocyte preprogramming and subsequent peripheral Treg cell differentiation
The nature of activation signals is essential in determining T cell subset differentiation; however, the features that determine T cell subset...
-
Semaphorin-3A Inhibits Proliferation, but Does Not Affect Apoptosis of Mouse Thymocytes In Vitro
Neuronal factor semaphorin-3A is viewed as immune suppressant of peripheral T lymphocytes, but it can also negatively affect activity of the thymus,...
-
Single-cell multiomic analysis of thymocyte development reveals drivers of CD4+ T cell and CD8+ T cell lineage commitment
The development of CD4 + T cells and CD8 + T cells in the thymus is critical to adaptive immunity and is widely studied as a model of lineage...
-
Light-Induced Functional Pinealectomy. Effect on the Thymus of C57BL/6 Mice
Light-induced functional pinealectomy was simulated in C57BL/6 mice by 14-day exposure to constant lighting. Immunophenoty** of CD3 hi and CD3 low ...
-
A double-negative thymocyte-specific enhancer augments Notch1 signaling to direct early T cell progenitor expansion, lineage restriction and β-selection
T cell differentiation requires Notch1 signaling. In the present study, we show that an enhancer upstream of Notch1 active in double-negative (DN)...
-
Effect of Melatonin on the Content of CD3low and CD3hi T Cells in the Thymus of Mice with Functional Pinealectomy
Continuous lighting for 14 days (functional pinealectomy model) leads to a decrease in the relative number of CD3 low and CD3 hi T lymphocytes and the...
-
Genetic Strategies to Study T Cell Development
Genetics approaches have been instrumental to deciphering T cell development in the thymus, including gene disruption by homologous recombination and...
-
Identification and Purification of Human T Cell Precursors
During their development, human T cells undergo similar genomic changes and pass through the same developmental checkpoints as develo** thymocytes...
-
A Simplified Approach to Evaluating T Cell Development by Flow Cytometry
T cell development is a complex multistep process that requires the coordinated activation of distinct signaling responses and the regulated...
-
Effect of Glycyrrhetic Acid Derivatives on Regulation of Thymocyte Volume
We studied the effects of glycyrrhetinic acid (bioactive aglycone of glycyrrhizin) and its ester derivatives at positions C-3 and C-30 on the cell...
-
Differences in Age-Related Changes in the Thymus in Rats Developmentally Exposed to Endocrine Disrupter Dichlorodiphenyltrichloroethane
We studied features of age-related changes in the thymus of mature male Wistar rats developmentally exposed to the endocrine disruptor...
-
In Vitro Analysis of Thymocyte Signaling
When a develo** thymocyte expresses a TCR, it is subjected to numerous interactions with self-peptide/MHC complexes that determine its fate. These...
-
The phosphatidylinositol-transfer protein Nir3 promotes PI(4,5)P2 replenishment in response to TCR signaling during T cell development and survival
Hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP 2 ) by phospholipase C-γ (PLCγ1) represents a critical step in T cell antigen receptor (TCR)...
-
Intrathymic dendritic cell-biased precursors promote human T cell lineage specification through IRF8-driven transmembrane TNF
The cross-talk between thymocytes and thymic stromal cells is fundamental for T cell development. In humans, intrathymic development of dendritic...
-
Fetal Thymic Organ Culture (FTOC) Optimized for Gamma-Delta T Cell Studies
Fetal thymic organ culture (FTOC) provides a method for analyzing T cell development in a physiological context outside the animal. This technique...