Abstract
During their development, human T cells undergo similar genomic changes and pass through the same developmental checkpoints as develo** thymocytes in the mouse. The difference between both species, however, is that some of these developmental stages are characterized by different phenotypic markers, and as a result, evidence emerges that the molecular regulation of human T cell development subtly differs from the mouse (Taghon et al., Curr Top Microbiol Immunol 360:75–97, 2021; Haddad et al., Immunity 24:217–230, 2006; Hao et al., Blood 111:1318–1326, 2008; Taghon and Rothenberg, Semin Immunopathol 30:383–398, 2008). In this chapter, we describe in detail how the different stages of human T cell development can be characterized and isolated using specific surface markers.
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References
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Van de Walle, I., Lambrechts, N., Derveeuw, A., Lavaert, M., Roels, J., Taghon, T. (2023). Identification and Purification of Human T Cell Precursors. In: Bosselut, R., Vacchio, M.S. (eds) T-Cell Development. Methods in Molecular Biology, vol 2580. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2740-2_20
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DOI: https://doi.org/10.1007/978-1-0716-2740-2_20
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-2739-6
Online ISBN: 978-1-0716-2740-2
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