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Open AccessThe Korean undiagnosed diseases program phase I: expansion of the nationwide network and the development of long-term infrastructure
Phase I of the Korean Undiagnosed Diseases Program (KUDP), performed for 3 years, has been completed. The Phase I program aimed to solve the problem of undiagnosed patients throughout the country and develop i...
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Article
Open AccessIQ-Switch is a QF-based innocuous, silencing-free, and inducible gene switch system in zebrafish
Though various transgene expression switches have been adopted in a wide variety of organisms for basic and biomedical research, intrinsic obstacles of those existing systems, including toxicity and silencing,...
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Article
Open AccessTransgenic fluorescent zebrafish lines that have revolutionized biomedical research
Since its debut in the biomedical research fields in 1981, zebrafish have been used as a vertebrate model organism in more than 40,000 biomedical research studies. Especially useful are zebrafish lines express...
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Article
Enzymatic construction of shRNA library from oligonucleotide library
Short hairpin RNAs (shRNAs) expressed from vectors have been used as an effective means of exploiting the RNA interference (RNAi) pathway in mammalian cells. Genome-scale screening with shRNA libraries has bee...
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Article
Promoter cross-talk affects the inducible expression of intronic shRNAs from the tetracycline response element
RNA interference (RNAi), defined as double-stranded, RNA-mediated gene silencing, is a useful tool for functional genomic studies. Along with increasing information about genomic sequences due to the innovativ...
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Article
Enhancement of gene knockdown efficiency by CNNC motifs in the intronic shRNA precursor
Short hairpin RNAs (shRNAs) expressed from vectors have been used as an effective means of exploiting the RNA interference (RNAi) pathway in mammalian cells. Of several methods to express shRNA, a method of tr...
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Article
Open AccessNanobody-targeted E3-ubiquitin ligase complex degrades nuclear proteins
Targeted protein degradation is a powerful tool in determining the function of specific proteins or protein complexes. We fused nanobodies to SPOP, an adaptor protein of the Cullin-RING E3 ubiquitin ligase com...
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Article
Animal models of amyotrophic lateral sclerosis and Huntington’s disease
Amyotrophic lateral sclerosis (ALS) and Huntington’s disease (HD) are debilitating neurodegenerative conditions for which there is no effective cure. Genetic determinants of both diseases have been identified,...
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Meeting report: genetics and genome engineering
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Tackling neurodegenerative diseases: animal models of Alzheimer’s disease and Parkinson’s disease
Our ageing society is confronted with a dramatic increase in incidence of age-related neurodegenerative diseases; biomedical research leading to novel therapeutic strategies is crucial to address this problem....
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Enzymatic production and expression of shRNAmir30 from cDNAs
RNA interference (RNAi) is an important tool for studying gene function and genetic networks. Double-stranded RNA (dsRNA) triggers RNAi that selectively silences gene expression mainly by degrading target mRNA...
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Article
Live image profiling of neural crest lineages in zebrafish transgenic lines
Zebrafish transgenic lines are important experimental tools for lineage tracing and imaging studies. It is crucial to precisely characterize the cell lineages labeled in transgenic lines to understand their li...
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Article
Genomic mismatch scanning: a new approach to genetic linkage map**
Genomic mismatch scanning (GMS) is a new method of genetic linkage analysis that does not require conventional polymorphic markers or gel electrophoresis. GMS is ideally suited to affected–relative–pair mappin...
