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Article
Open AccessEngineering and evaluation of FXa bypassing agents that restore hemostasis following Apixaban associated bleeding
Direct oral anticoagulants (DOACs) targeting activated factor Xa (FXa) are used to prevent or treat thromboembolic disorders. DOACs reversibly bind to FXa and inhibit its enzymatic activity. However, DOAC trea...
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Article
Open AccessInvestigation of thrombin concentration at the time of clot formation in simultaneous thrombin and fibrin generation assays
Thrombin generation (TG) and fibrin clot formation represent the central process of blood coagulation. Up to 95% of thrombin is considered to be generated after the clot is formed. However, this was not invest...
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Article
Open AccessA structural homology approach to identify potential cross-reactive antibody responses following SARS-CoV-2 infection
The emergence of the novel SARS-CoV-2 virus is the most important public-health issue of our time. Understanding the diverse clinical presentations of the ensuing disease, COVID-19, remains a critical unmet ne...
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Article
Open AccessModified aptamers as reagents to characterize recombinant human erythropoietin products
Reliable and reproducible monitoring of the conformational state of therapeutic protein products remains an unmet technological need. This need is amplified by the increasing number of biosimilars entering the...
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Article
Open AccessQuantum-chemical, NMR, FT IR, and ESI MS studies of complexes of colchicine with Zn(II)
Colchicine is a tropolone alkaloid from Colchicinum autumnale. It shows antifibrotic, antimitotic, and anti-inflammatory activities, and is used to treat gout and Mediterranean fever. In this work, complexes of c...
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Article
Cyclophilin A promotes cell migration via the Abl-Crk signaling pathway
Cyclophilin A binds a proline motif in human CrkII, preventing phosphorylation by Abl and EGFR. Decreased CrkII phosphorylation ensures interactions with the focal adhesion proteins paxillin and p130CAS to sti...
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Article
Computational design of ligand-binding proteins with high affinity and selectivity
Computational protein design is used to create a protein that binds the steroid digoxigenin (DIG) with high affinity and selectivity; the computational design methods described here should help to enable the d...
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Article
Domain organization differences explain Bcr-Abl's preference for CrkL over CrkII
NMR structures of CrkL, an adaptor protein that mediates Bcr-Abl signaling in CML, reveal domain organization distinct from CrkII that allows constitutive interaction between CrkL and Abl kinase.
