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Article
HCO 3 - and OH-transport across the plasmalemma ofChara
Internodal and whorl (branch) cells of the green alga,Chara corallina Klein ex Willd., em. R.D.W., were studied with the extracellular vibrating probe for measuring transmembrane ion currents, and with an extrace...
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Article
The relationship of the charasome to chloride uptake in Chara corallina: physiological and histochemical investigations
A possible role of the charasome in terms of chloride transport into Chara corallina Klein ex. Willd., em. R.D.W. is examined. The branches of Chara contain the most charasome material and are shown to be very ef...
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Article
Mechanism of acquisition of exogenous bicarbonate by internodal cells of Chara corallina
Electrophysiological measurements on internodal cells of the alga, Chara corallina Klein ex Willd., em. R.D.W., showed that the potential across the plasmalemma was sensitive to the level of exogenous HCO ...
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Article
Ultrastructural and histochemical studies on guard cells
Serial thick sections of guard cells from Vicia faba L., Nicotiana tabacum L., Allium cepa L., Zea mays L. and Beta vulgaris L. were obtained systematically (600–800 nm) and viewed with the transmission electron ...
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Article
Sugar uptake into Allium cepa leaf tissue: an integrated approach
Allium cepa L. leaves were subjected to enzymatic (pectolyase) and mechanical manipulation in order to ascertain the contribution made by various leaf tissues to the total sugar uptake by the leaf. In order to d...
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Chapter
Ion Transport Processes in Corn Roots: An Approach Utilizing Microelectrode Techniques
There have been many studies conducted on ion or salt absorption by plant roots. This research has focused both on the basic mechanisms involved in transporting the ion into the plant and on the mineral nutrie...
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Article
Control of photoassimilate movement in source-leaf tissues of Ipomoea tricolor Cav.
Autoradiographs obtained from discs from mature, peeled Ipomoea tricolor Cav. leaves indicated that a 15-min 2 mM p−chloromercuribenzene sulfonic acid (PCMBS) pretreatment did not prevent the accumulation of 14CO
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Article
Effects of microtubule agents on the spatial and electrical properties of the plasma membrane inChara corallina
The freshwater algaChara corallina Klein ex Willd., em. R.D.W. (=C. australis R.Br.) develops alternating outward (acid) and inward (alkaline) current areas on its surface when illuminated. Exposure of cells to v...
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Article
Direct measurement of the reversal potential and current-voltage characteristics in the acid and alkaline regions of Chara corallina
A new vibrating probe-current voltage measuring system is described which enabled us to detect current-voltage curves in the acid and alkaline regions of Chara corallina (Klein ex Willd., em. R.D.W.). Extracellul...
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Article
Use of an extracellular, ion-selective, vibrating microelectrode system for the quantification of K+, H+, and Ca2+ fluxes in maize roots and maize suspension cells
An ion-selective vibrating-microelectrode system, which was originally used to measure extracellular Ca2+ gradients generated by Ca2+ currents, was used to study K+, H+ and Ca2+ transport in intact maize (Zea may...
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Article
Influence of the tobacco mosaic virus 30-kDa movement protein on carbon metabolism and photosynthate partitioning in transgenic tobacco plants
Transgenic tobacco (Nicotiana tabacum L.) plants expressing the 30-kDa movement protein of tobacco mosaic virus (TMV-MP) were employed to investigate the influence of a localized change in mesophyll-bundle sheath...
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Article
Mechanism of plasmodesmata formation in characean algae in relation to evolution of intercellular communication in higher plants
It is generally accepted that higher plants evolved from ancestral forms of the modern charophytes. For this reason, we chose the characean alga, Chara corallina Klein ex Willd., em. R.D.W. (C. australis R. Br.),...
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Article
Establishment of a cell-to-cell communication pathway between separate carpels during gynoecium development
In the evolutionarily advanced angiosperm flower, postgenital fusion is often involved in the formation of the female reproductive organ, the gynoecium. In the present study, we report on the early establishme...
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Article
Pleiotropic effects of tobacco-mosaic-virus movement protein on carbon metabolism in transgenic tobacco plants
Transgenic tobacco (Nicotiana tabacum L. cv. Xanthi) plants expressing wild-type or mutant forms of the 30-kDa movement protein of tobacco mosaic virus (TMV-MP) were employed to study the effects of the TMV-MP on...
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Article
Characterization and manipulation of embryogenic response from in-vitro-cultured immature inflorescences of rice (Oryza sativa L.)
Characterization and optimization of the embryogenic response from in-vitro-cultured immature inflorescences of rice (Oryza sativa L. sub-species indica and japonica) are described. Histological and morphological...
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Article
The molecular basis of potassium nutrition in plants
Over the last five years, the cloning and characterization of K+ transport genes corresponding to K+ channels (KAT1, AKT1, KST1, AKT2), associated subunits (KAB1) and a high-affinity transporter (HKT1) has opened...
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Article
Application of microinjection techniques to plant nutrition
To gain a full understanding of the complex processes that underlie plant nutrition requires the elucidation of the genetic, molecular, biochemical, biophysical, physiological and environmental factors that in...
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Article
Rice phloem thioredoxin h has the capacity to mediate its own cell-to-cell transport through plasmodesmata
Rice (Oryza sativa L.) phloem sieve tubes contain RPP13-1, a thioredoxin h protein that moves around the plant via the translocation stream. Such phloem-mobile proteins are thought to be synthesized in the compa...
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Article
Identification of immunologically related proteins in sieve-tube exudate collected from monocotyledonous and dicotyledonous plants
The mature, functional sieve-tube system in higher plants is dependent upon protein import from the companion cells to maintain a functional long-distance transport system. Soluble proteins present within the...
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Article
Parallels between nuclear-pore and plasmodesmal trafficking of information molecules