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Article
Lunar gravity affects leaf movement of Arabidopsis thaliana in the International Space Station
Cyclic leaf ascent and descent occur in synchrony and phase congruence with the lunisolar tidal force under a broad range of conditions.
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Protocol
High-Throughput Phenoty** of Root Growth Dynamics
Plant organ phenoty** by noninvasive video imaging techniques provides a powerful tool to assess physiological traits, circadian and diurnal rhythms, and biomass production. In particular, growth of individu...
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Article
Open AccessQuantitative expression analysis of selected transcription factors in pavement, basal and trichome cells of mature leaves from Arabidopsis thaliana
Gene expression levels of several transcription factors from Arabidopsis thaliana that were described previously to be involved in leaf development and trichome formation were analysed in trichome, basal and pave...
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Article
Expression of a Petunia inflata pectin methyl esterase in Solanum tuberosum L. enhances stem elongation and modifies cation distribution
Transgenic potato (Solanum tuberosum L.) plants were constructed with a Petunia inflata-derived cDNA encoding a pectin methyl esterase (PME; EC 3.1.1.11) in sense orientation under the control of the cauliflower...
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Article
Gas-exchange analysis of chloroplastic fructose-1,6-bisphosphatase antisense potatoes at different air humidities and at elevated CO2
Gas-exchange measurements were performed to analyze the leaf conductances and assimilation rates of potato (Solanum tuberosum L. cv. Desireé) plants expressing an antisense construct against chloroplastic fructo...
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Article
Analysis of phloem protein patterns from different organs of Cucurbita maxima Duch. by matrix-assisted laser desorption/ionization time of flight mass spectroscopy combined with sodium dodecyl sulfate-polyacrylamide gel electrophoresis
Sieve tubes mediate the long-distance transport of nutrients and signals between source and sink organs of plants. To detect mobile phloem proteins that are differentially distributed in source and sink organ...
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Article
Remote stimulation by heat induces characteristic membrane-potential responses in the veins of wild-type and abscisic acid-deficient tomato plants
The systemic induction of proteinase inhibitor genes in tomato plants is either mediated by fast electrical signals or alternatively by chemical messengers. In the present study we analyzed the pathway of the...
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Chapter
Gas Exchange and Environmental Parameters at FBPase Antisense Potatoes
It has been frequently shown that stomatal conductance responded to changes in ambient CO2 concentration (1,2). On the other hand insensitivity was observed under special conditions (1,2). Reduction of leaf condu...
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Chapter
Exogenous electrical stimulation of tomato plants: Induction of proteinase inhibitor II gene expression and control of photosynthetic activity
Electrical and hydraulic signals have been identified as physical signals that mediate the systemic expression of wound inducible genes (1,2). The appearance of variation potentials and fast transient voltage ...
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Article
Direct measurement of the reversal potential and current-voltage characteristics in the acid and alkaline regions of Chara corallina
A new vibrating probe-current voltage measuring system is described which enabled us to detect current-voltage curves in the acid and alkaline regions of Chara corallina (Klein ex Willd., em. R.D.W.). Extracellul...
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Article
Effects of microtubule agents on the spatial and electrical properties of the plasma membrane inChara corallina
The freshwater algaChara corallina Klein ex Willd., em. R.D.W. (=C. australis R.Br.) develops alternating outward (acid) and inward (alkaline) current areas on its surface when illuminated. Exposure of cells to v...
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Article
Inversion of extracellular current and axial voltage profile inChara andNitella
Reducing the pH of the bathing solution from 8.2 to pH 6 can induce an inversion of the extracellular current pattern that develops at the surface ofChara corallina internodal cells. A similar result can be obtai...
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Article
I/V-Curve studies of the control of a K+ transporter inNitella by temperature
InNitella, current-voltage relationships were measured at different temperatures ranging from 5 to 25°C. Sets of theseI/V curves were subject to curve fitting on the basis of a cyclic reaction scheme (Class I mod...
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Article
Determination of charge, stoichiometry and reaction constants fromI–V curve studies on a K+ transporter inNitella
InNitella cells with low pump activity, the electrical characteristics of membrane transport are mainly determined by K+ transport. Current-voltage curves were measured at outside K+ concentrations ranging from 0...