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Chapter
A Guide to Preclinical Models of Zoster-Associated Pain and Postherpetic Neuralgia
Reactivation of latent varicella-zoster virus (VZV) causes herpes zoster (HZ), which is commonly accompanied by acute pain and pruritus over the time course of a zosteriform rash. Although the rash and associa...
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Article
Open AccessGlioblastoma infiltration of both tumor- and virus-antigen specific cytotoxic T cells correlates with experimental virotherapy responses
The mode of action for oncolytic viruses (OVs) in cancer treatment is thought to depend on a direct initial cytotoxic effect against infected tumor cells and subsequent activation of immune cell responses dire...
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Protocol
Engineering HSV-1 Vectors for Gene Therapy
Virus vectors have been employed as gene transfer vehicles for various preclinical and clinical gene therapy applications and with the approval of Glybera (Alipogene tiparvovec) as the first gene therapy produ...
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Article
Open AccessCurrent gene therapy using viral vectors for chronic pain
The complexity of chronic pain and the challenges of pharmacotherapy highlight the importance of development of new approaches to pain management. Gene therapy approaches may be complementary to pharmacotherap...
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Protocol
Engineering HSV-1 Vectors for Gene Therapy
Virus vectors have been employed as gene transfer vehicles for various preclinical and clinical gene therapy applications, and with the approval of Glybera (alipogene tiparvovec) as the first gene therapy prod...
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Article
Correction: Progress in gene therapy for neurological disorders
Nat. Rev. Neurol. 9, 277–291 (2013); doi:10.1038/nrneurol.2013.56 In the version of this article initially published, HSV-1‒thymidine kinase was incorrectly referred to as tyrosine kinase in Table 4, and as HS...
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Article
Progress in gene therapy for neurological disorders
Neurological diseases that result from an underlying genetic mutation or from acquired genetic and/or epigenetic changes are prevalent in the population, and might be amenable to gene therapy. In this Review, ...
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Article
Varicella zoster virus-induced pain and post-herpetic neuralgia in the human host and in rodent animal models
Pain and post-herpetic neuralgia (PHN) are common and highly distressing complications of herpes zoster that remain a significant public health concern and in need of improved therapies. Zoster results from re...
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Protocol
Construction and Production of Recombinant Herpes Simplex Virus Vectors
Virus vectors have been employed as gene transfer vehicles for various pre-clinical and clinical gene therapy applications. Replication-competent herpes simplex virus (HSV) vectors that replicate specifically ...
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Article
A new transgene reporter for in vivo magnetic resonance imaging
We report a new platform technology for visualizing transgene expression in living subjects using magnetic resonance imaging (MRI). Using a vector, we introduced an MRI reporter, a metalloprotein from the ferr...
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Protocol
Gene Transfer to Glial Tumors Using Herpes Simplex Virus
Glial tumors occur as intraaxial masses in the brain and are uniformly fatal due to lack of effective therapy. Resection combined with radiation and chemotherapy fails to eradicate malignant cells infiltrating...
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Protocol
Delivery of Herpes Simplex Virus-Based Vectors to Stem Cells
In contrast to traditional drugs that generally act by altering existing gene product function, gene therapy aims to target the root cause of the disease by altering the genetic makeup of the cell to treat the...
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Protocol
Delivery Using Herpes Simplex Virus
The human herpesviruses represent excellent candidate viruses for several types of gene vector applications. As a class, they are large DNA viruses with the potential to accommodate large or multiple transgene...
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Protocol
Use of the Herpes Simplex Viral Genome to Construct Gene Therapy Vectors
Herpes simplex virus (HSV) is an enveloped double-stranded DNA virus (see Fig. 1A-reviewed in ref. 1). The mature virion consists of the following components: ...
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Protocol
Development of Replication-Defective Herpes Simplex Virus Vectors
A greater understanding of the molecular, biochemical, and genetic factors involved in the progression of a specific disease state has led to the development of genetic therapies using direct gene transfer to ...
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Protocol
Development of Replication-Defective Herpes Simplex Virus Vectors
Numerous diseases of the nervous system result from single gene or multifactorial gene defects such as cancer, immune pathological disorders, metabolic diseases, and common neurodegenerative syndromes (Parkins...
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Article
HSV as a gene transfer vector for the nervous system
Gene therapy for diseases of the nervous system requires vectors capable of delivering the therapeutic gene into postmitotic cells in vivo. Herpes simplex virus type 1 is a neurotropic virus that naturally est...
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Chapter
Neurovirulence of Herpes Simplex Virus Type 1 Accessory Gene Mutants
A striking feature of the genome of herpes simplex virus (HSV) is that approximately half of the genes are not essential for replication of the virus in cell culture. It might be predicted that many of these a...
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Chapter
Development of Herpes Simplex Virus Vectors for Gene Transfer to the Central Nervous System
Advances in understanding the molecular basis of human disease in the past decade have led to the identification and cloning of genes responsible for certain heritable diseases of the central nervous system (C...
