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Article
Open AccessKnowledge about age-related eye diseases in the general population in Germany
With a rising prevalence of age-related eye diseases, prevention and early diagnosis of these conditions are key goals of public eye health. Disease-related knowledge in the general public supports these goals...
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Article
Open AccessPost-transcriptional regulation of GABAB receptor and GIRK1 channels by Nogo receptor 1
Type B GABA receptors (GABA Rs) play a critical role in synaptic transmission. We carried out studies to determine whether neuronal cell surface expression of GABAB-Rs might be regulated by the Nogo receptor 1...
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Article
Correction: Progress in gene therapy for neurological disorders
Nat. Rev. Neurol. 9, 277–291 (2013); doi:10.1038/nrneurol.2013.56 In the version of this article initially published, HSV-1‒thymidine kinase was incorrectly referred to as tyrosine kinase in Table 4, and as HS...
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Article
Progress in gene therapy for neurological disorders
Neurological diseases that result from an underlying genetic mutation or from acquired genetic and/or epigenetic changes are prevalent in the population, and might be amenable to gene therapy. In this Review, ...
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Reference Work Entry In depth
Opioids and Gene Therapy
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Article
Open AccessReduction of voltage gated sodium channel protein in DRG by vector mediated miRNA reduces pain in rats with painful diabetic neuropathy
Painful neuropathy is a common complication of diabetes. Previous studies have identified significant increases in the amount of voltage gated sodium channel isoforms NaV1.7 and NaV1.3 protein in the dorsal root ...
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Article
Open AccessGlial TNFα in the spinal cord regulates neuropathic pain induced by HIV gp120 application in rats
HIV-associated sensory neuropathy (HIV-SN) is one of the most common forms of peripheral neuropathy, affecting about 30% of people with acquired immune deficiency syndrome (AIDS). The symptoms of HIV-SN are do...
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Article
The Role of TNFα in the Periaqueductal Gray During Naloxone-Precipitated Morphine Withdrawal in Rats
Tolerance and dependence are common complications of long-term treatment of pain with opioids, which substantially limit the long-term use of these drugs. The mechanisms underlying these phenomena are poorly u...
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Article
Gene therapy for the treatment of diabetic neuropathy
Neuropathy is a common, untreatable complication of type 1 and type 2 diabetes. In animal models peptide neurotrophic factors can be used to protect against the development of neuropathy, but the combination o...
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Reference Work Entry In depth
Opioids and Gene Therapy
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Article
Open AccessHSV-mediated expression of interleukin-4 in dorsal root ganglion neurons reduces neuropathic pain
To examine the role of inflammatory mediators in neuropathic pain, we used a replication-defective genomic herpes simplex virus (HSV)-based vector containing the coding sequence for the anti-inflammatory pepti...
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Protocol
Use of Laser Capture Microdissection Together With In Situ Hybridization and Real-Time PCR to Study Distribution of Latent Herpes Simplex Virus Genomes in Mouse Trigeminal Ganglia
The herpes simplex virus (HSV) frequently establishes a latent state in neurons, which can then be reactivated from the infected neurons. Quantifying the single-cell viral load is essential for understanding l...
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Protocol
Delivery of Herpes Simplex Virus-Based Vectors to the Nervous System
Gene transfer to the nervous system is an attractive option to treat a wide variety of neurological insults (1–3). The expression of trophic factor and/or antiapoptotic genes may be beneficial in halting the slow...
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Protocol
Delivery of Herpes Simplex Virus-Based Vectors to Stem Cells
In contrast to traditional drugs that generally act by altering existing gene product function, gene therapy aims to target the root cause of the disease by altering the genetic makeup of the cell to treat the...
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Protocol
Delivery Using Herpes Simplex Virus
The human herpesviruses represent excellent candidate viruses for several types of gene vector applications. As a class, they are large DNA viruses with the potential to accommodate large or multiple transgene...
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Article
Therapeutic gene transfer to the nervous system using viral vectors
The past few years have been marked by substantial progress in preclinical studies of therapeutic gene transfer for neurologic disease using viral-based vectors. In this article, the authors review the data re...
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Article
The relationship of herpes simplex virus latency associated transcript expression to genome copy number: A quantitative study using laser capture microdissection
To investigate the quantitative relationship of latent herpes simplex virus (HSV) genomes to the expression of latency associated transcripts (LATs) we used a combination of laser capture microdissection (LCM)...
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Protocol
Development of Replication-Defective Herpes Simplex Virus Vectors
A greater understanding of the molecular, biochemical, and genetic factors involved in the progression of a specific disease state has led to the development of genetic therapies using direct gene transfer to ...
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Article
HveC (nectin-1) is expressed at high levels in sensory neurons, but not in motor neurons, of the rat peripheral nervous system
The entry of herpes simplex virus (HSV)-1 into cells is a complex process mediated in part by the binding of the HSV glycoprotein D (gD) to a specific cellular receptor identified as HveC, or nectin-1. We exam...
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Protocol
Analysis of HSV-DNA and RNA Using the Polymerase Chain Reaction
The polymerase chain reaction (PCR) technique is a sensitive method for detection of nucleic acids that can be used to detect herpes simplex virus (HSV)-DNA and RNA in tissue samples with greater sensitivity t...
