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Article
Open AccessIntermolecular base stacking mediates RNA-RNA interaction in a crystal structure of the RNA chaperone Hfq
The RNA-chaperone Hfq catalyses the annealing of bacterial small RNAs (sRNAs) with target mRNAs to regulate gene expression in response to environmental stimuli. Hfq acts on a diverse set of sRNA-mRNA pairs us...
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Article
Open AccessA bromodomain–DNA interaction facilitates acetylation-dependent bivalent nucleosome recognition by the BET protein BRDT
Bromodomains are critical components of many chromatin modifying/remodelling proteins and are emerging therapeutic targets, yet how they interact with nucleosomes, rather than acetylated peptides, remains uncl...
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Article
Structural basis of histone H2A–H2B recognition by the essential chaperone FACT
The crystal structure of the FACT histone chaperone domain Spt16M in complex with the H2A–H2B heterodimer is solved; Spt16M makes several interactions with histones and seems to block the interaction of H2B wi...
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Article
A family of macrodomain proteins reverses cellular mono-ADP-ribosylation
ADP-ribosylation catalyzed by PARPs and sirtuins is an important post-translation modification. Macrodomain proteins MacroD1 and D2 are now shown to preferentially bind mono-ADP-ribosylated proteins and to act...
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Article
Multi-domain conformational selection underlies pre-mRNA splicing regulation by U2AF
Different domains of a protein can interact dynamically to recognize a ligand, but the coordination of binding by multiple domains is not well understood. Michael Sattler and colleagues have investigated bindi...
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Article
68Ga-labelled recombinant antibody variants for immuno-PET imaging of solid tumours
Recombinant antibodies isolated from human antibody libraries have excellent affinities and high target specificity. As full-length IgGs are cleared inadequately slowly from the circulation, the aim of this wo...
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Article
Computer-aided design of a PDZ domain to recognize new target sequences
PDZ domains are small globular domains that recognize the last 4–7 amino acids at the C-terminus of target proteins. The specificity of the PDZ–ligand recognition is due to side chain–side chain interactions, ...
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Article
Functional organization of the yeast proteome by systematic analysis of protein complexes
Most cellular processes are carried out by multiprotein complexes. The identification and analysis of their components provides insight into how the ensemble of expressed proteins (proteome) is organized into ...
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Article
The Eps8 protein coordinates EGF receptor signalling through Rac and trafficking through Rab5
How epidermal growth factor receptor (EGFR) signalling is linked to EGFR trafficking is largely unknown. Signalling and trafficking involve small GTPases of the Rho and Rab families, respectively. But it remai...
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Article
Crystal structure of the protein disulfide bond isomerase, DsbC, from Escherichia coli
DsbC is one of five Escherichia coli proteins required for disulfide bond formation and is thought to function as a disulfide bond isomerase during oxidative protein folding in the periplasm. DsbC is a 2 × 23 kDa...
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Article
Endosome dynamics regulated by a Rho protein
Vesicular transport is a dynamic process that requires coordinated interactions between membrane and cytoskeleton. The mechanisms and molecules integrating these interactions are unclear. A Rho protein, RhoD, ...
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Article
GTPase activity of Rab5 acts as a timer for endocytic membrane fusion
THE GTPase cycle is a versatile regulatory mechanism directing many cell functions1, and Rab family members use it to regulate intracellular transport2–4. Current models propose that GTP hydrolysis by Rab protein...
