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Article
Open AccessDynamic arrest and aging of biomolecular condensates are modulated by low-complexity domains, RNA and biochemical activity
Biomolecular condensates require suitable control of material properties for their function. Here we apply Differential Dynamic Microscopy (DDM) to probe the material properties of an in vitro model of process...
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Article
Open AccessCharacterization of RNA content in individual phase-separated coacervate microdroplets
Condensates formed by complex coacervation are hypothesized to have played a crucial part during the origin-of-life. In living cells, condensation organizes biomolecules into a wide range of membraneless compa...
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Protocol
Probing Liquid–Liquid Phase Separation of RNA-Binding Proteins In Vitro and In Vivo
Biomolecular condensates and the concept of liquid–liquid phase separation (LLPS) have transformed cell biology in recent years. Condensates organize cellular content and compartmentalize biochemical reactions...
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Article
DEAD-box ATPases are global regulators of phase-separated organelles
The ability of proteins and nucleic acids to undergo liquid–liquid phase separation has recently emerged as an important molecular principle of how cells rapidly and reversibly compartmentalize their component...
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Article
Structural basis of histone H2A–H2B recognition by the essential chaperone FACT
The crystal structure of the FACT histone chaperone domain Spt16M in complex with the H2A–H2B heterodimer is solved; Spt16M makes several interactions with histones and seems to block the interaction of H2B wi...
