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Chapter
Chemical and Enzymatic Synthesis and Production of Glycans
Oligosaccharide, Glycoconjugates, Glycosylation, Stereoselectivity
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Article
Chemo-enzymatic synthesis of a glycosylated peptide containing a complex N-glycan based on unprotected oligosaccharides by using DMT-MM and Endo-M
For chemo-enzymatic synthesis of a glycosylated peptide, 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMT-MM) was used for the synthesis of a N-acetylglucosaminyl peptide and a pseudoglycop...
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Chapter
Interaction Assay of Oligosaccharide with Protein Using Fluorescence Polarization (FP) and Fluorescence Correlation Spectroscopy (FCS)
Carbohydrate-protein interaction is very important in understanding the function of sugar chains. The immobilization or fluorescence modification of oligosaccharides was investigated to understand the biologic...
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Chapter
Chemo-Enzymatic Synthesis of Glycoconjugates
Synthesis of glycoconjugate is one of the most important difficulties in carbohydrate chemistry. Although many chemical synthetic methods have been developed, the synthesis of glycoconjugates with natural glyc...
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Article
Self-aggregation of synthetic zinc chlorophyll derivatives possessing multi-perfluoroalkyl chains in perfluorinated solvents
Zinc 31-hydroxy-131-oxo-chlorins possessing two, three, four and six perfluorooctyl chains were synthesized from naturally occurring chlorophyll-a. Only the synthetic zinc chlorin possessing six perfluorooctyl ch...
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Article
A chemical synthesis of UDP-LacNAc and its regioisomer for finding ‘oligosaccharide transferases’
A chemical synthesis of uridine 5’-diphospho-N-acetyllactosamine (Galβ(1→4)GlcNAc-UDP; UDP-LacNAc) and Galβ(1→3)GlcNAc-UDP is described. Coupling of the disaccharide imidate derivatives with dibenzylphosphate gav...
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Article
Saccharide-branched Cyclodextrins as Targeting Drug Carriers
A synthetic series of heptakis-galactose-branched cyclodextrins (termed CDs) having a longer spacer arm using two amino-caproic acids as an enlarging unit were prepared. Starting with heptakis-amino-β-CD or hepta...
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Article
Chemo-enzymatic synthesis and structure-activity study of artificially N-glycosylated eel calcitonin derivatives with a complex type oligosaccharide
Starting from N-glycosylated eel calcitonin derivatives that contain an N-acetyl-D-glucosamine residue specifically at the 3rd, 14th, 20th or 26th amino acid residue, corresponding glycopeptides with a complex-ty...
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Chapter
Chemo-enzymatic syntheses of eel calcitonin analogs having natural N-linked oligosaccharides
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Article
Effect of carbohydrate structure on biological activity of artificially N-glycosylated eel calcitonin
To reveal the function of the carbohydrate portion of glycopeptides and glycoproteins, we chemo-enzymatically synthesized artificially N-glycosylated derivatives of eel calcitonin and studied their three-dimensio...
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Chapter and Conference Paper
The Synthesis of Oligosaccharide-Branched Cyclodextrins
Cyclodextrins (CDs) can be used in various drugs as an auxiliary additive such as a carrier, diluent, and solubilizer for tablet ingredients.1–3 The syntheses of natural oligosaccharide-branched CDs, which showed...
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Article
The synthesis of oligosaccharide-branched cyclodextrins and their interaction with concanavalin A
New oligosaccharide-branched β-cyclodextrins having a various oligosaccharides in a primary hydroxyl group of β-cyclodextrin(CD) were synthesized and examined for the interaction with the immobilized concanava...
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Chapter and Conference Paper
The Synthesis of Oligosaccharide-Branched Cyclodextrins and their Interaction with Concanavalin A
New oligosaccharide-branched β-cyclodextrins having a various oligosaccharides in a primary hydroxyl group of β-cyclodextrin(CD) were synthesized and examined for the interaction with the immobilized concanava...
