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Interaction Assay of Oligosaccharide with Protein Using Fluorescence Polarization (FP) and Fluorescence Correlation Spectroscopy (FCS)

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Experimental Glycoscience

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Abstract

Carbohydrate-protein interaction is very important in understanding the function of sugar chains. The immobilization or fluorescence modification of oligosaccharides was investigated to understand the biological interaction between various species and oligosaccharides. In many cases of immobilization and modification of carbohydrates, the structure of the glycan was found to be relatively small, because the synthesis of an oligosaccharide is not easy compared with that of an oligopeptide or oligonucleotide.

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References

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Author information

Authors and Affiliations

  1. Department of Applied Chemistry, School of Engineering, and Institute of Glycotechnology, Tokai University, 1117 Kitakaname, Hiratsuka, Kanagawa, 259-1292, Japan

    Toshiyuki Inazu

Authors
  1. Toshiyuki Inazu
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Editors and Affiliations

  1. Department of Disease Glycomics, Research Institute for Microbial Diseases, Osaka University, 1-1 Yamadaoka, Suita, Osaka, 565-0871, Japan

    Naoyuki Taniguchi M.D., Ph.D. (Professor) (Professor)

  2. Systems Glycobiology Group, Advanced Science Institute, RIKEN, Wako, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan

    Naoyuki Taniguchi M.D., Ph.D. (Professor) (Professor)

  3. Institute of Glycotechnology, Future Science and Technology Joint Research Center, Tokai University, Hiratsuka, Kanagawa, 259-1292, Japan

    Akemi Suzuki M.D., Ph.D. (Professor) (Professor)

  4. RIKEN Discovery Research Institute, Synthetic Cellular Chemistry Laboratory, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan

    Yukishige Ito Ph.D. (Chief Researcher) (Chief Researcher)

  5. Research Center for Medical Glycoscience, Advanced Industrial Science and Technology, AIST Tsukuba, Central 2, Tsukuba, Ibaraki, 305-8568, Japan

    Hisashi Narimatsu M.D., Ph.D.

  6. Research Center for Glycobiotechnology, Ritsumeikan University, 1-1-1 Noji-Higashi, Kusatsu, Shiga, 525-8577, Japan

    Toshisuke Kawasaki Ph.D. (Professor) (Professor)

  7. Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka, 560-0043, Japan

    Sumihiro Hase Ph.D. (Professor) (Professor)

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Inazu, T. (2008). Interaction Assay of Oligosaccharide with Protein Using Fluorescence Polarization (FP) and Fluorescence Correlation Spectroscopy (FCS). In: Taniguchi, N., Suzuki, A., Ito, Y., Narimatsu, H., Kawasaki, T., Hase, S. (eds) Experimental Glycoscience. Springer, Tokyo. https://doi.org/10.1007/978-4-431-77924-7_36

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