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Article
Glycolipid analyses of light-harvesting chlorosomes from envelope protein mutants of Chlorobaculum tepidum
Chlorosomes are large and efficient light-harvesting organelles in green photosynthetic bacteria, and they characteristically contain large numbers of bacteriochlorophyll c, d, or e molecules. Self-aggregated bac...
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Article
Open AccessRhodobacter sphaeroides mutants overexpressing chlorophyllide a oxidoreductase of Blastochloris viridis elucidate functions of enzymes in late bacteriochlorophyll biosynthetic pathways
In previous studies we have demonstrated that chlorophyllide a oxidoreductases (CORs) from bacteriochlorophyll (BChl) a-producing Rhodobacter species and BChl b-producing Blastochloris viridis show distinct subst...
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Article
Isolation and characterization of a new bacteriochlorophyll-c bearing a neopentyl substituent at the 8-position from the bciD-deletion mutant of the brown-colored green sulfur bacterium Chlorobaculum limnaeum
We recently constructed the mutant of the brown-colored green sulfur bacterium Chlorobaculum limnaeum lacking BciD which was responsible for formation of a formyl group at the 7-position in bacteriochlorophyll(BC...
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Article
Isolation and structural determination of C8-vinyl-bacteriochlorophyll d from the bciA and bchU double mutant of the green sulfur bacterium Chlorobaculum tepidum
The mutant lacking enzymes BciA and BchU, that catalyzed reduction of the C8-vinyl group and methylation at the C20 position of bacteriochlorophyll (BChl) c, respectively, in the green sulfur bacterium Chlorobacu...
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Article
Open AccessDark-operative protochlorophyllide oxidoreductase generates substrate radicals by an iron-sulphur cluster in bacteriochlorophyll biosynthesis
Photosynthesis converts solar energy to chemical energy using chlorophylls (Chls). In a late stage of biosynthesis of Chls, dark-operative protochlorophyllide (Pchlide) oxidoreductase (DPOR), a nitrogenase-lik...
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Article
Bacteriochlorophyll homolog compositions in the bchU mutants of green sulfur bacteria
Chlorosomes of the green sulfur bacterium Chlorobaculum limnaeum contain a large number of self-aggregated bacteriochlorophyll (BChl) e molecules. The ΔbchU mutant of this organism lacks BchU, a C20-methyltransfe...
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Article
A variety of glycolipids in green photosynthetic bacteria
The compositions of glycolipids in the following seven strains of green photosynthetic bacteria were investigated at the molecular level using LC–MS coupled with an evaporative light scattering detector: Chlorobi...
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Article
Open AccessAn unexpectedly branched biosynthetic pathway for bacteriochlorophyll b capable of absorbing near-infrared light
Chlorophyllous pigments are essential for photosynthesis. Bacteriochlorophyll (BChl) b has the characteristic C8-ethylidene group and therefore is the sole naturally occurring pigment having an absorption maximum...
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Article
Open AccessA seventh bacterial chlorophyll driving a large light-harvesting antenna
The discovery of new chlorophyllous pigments would provide greater understanding of the mechanisms and evolution of photosynthesis. Bacteriochlorophyll f has never been observed in nature, although this name was ...
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Article
The pigment stoichiometry in a chlorophyll a/c type photosynthetic antenna
The trimeric fucoxanthin–chlorophyll a/c protein (FCP) was purified from a Japanese brown alga, Cladosiphon okamuranus TOKIDA. Its pigment stoichiometry was determined to be chlorophyll (Chl) a:Chl c ...
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Article
In vitro synthesis and characterization of bacteriochlorophyll-f and its absence in bacteriochlorophyll-e producing organisms
Bacteriochlorophyll(BChl)-f which has not yet been found in natural phototrophs was prepared by chemically modifying chlorophyll-b. The retention time of reverse-phase high-performance liquid chromatography of th...
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Article
Synthesis of 18O-labeled photosynthetically active chlorophylls at the 3- or 7-carbonyl group with high regioselectivity
The 3- and 7-formyl groups of chlorophyll-d (Chl-d) and bacteriochlorophyll-e (BChl-e), respectively, were regioselectively labeled with an isotopically stable oxygen-18 (18O) atom to give 31-18O-labeled Chl-d an...
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Article
X-ray crystal structure of the light-independent protochlorophyllide reductase
Some photosynthetic organisms — pine seedlings and other gymnosperms for instance — are capable of 'greening' in the dark, in contrast to the strict requirement of light for the greening of angiosperm seedling...
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Article
Accumulation of chlorophyllous pigments esterified with the geranylgeranyl group and photosynthetic competence in the CT2256-deleted mutant of the green sulfur bacterium Chlorobium tepidum
Green sulfur bacteria contain chlorophyllous pigments, chlorophyll (Chl) aPD and bacteriochlorophyll (BChl) aP, esterified with Δ2,6-phytadienol and phytol, respectively, which would be produced by reduction of t...
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Article
Identification of 3,4-didehydrorhodopin as major carotenoid in Rhodopseudomonas species
Recently we isolated the purple photosynthetic bacterium, Rhodopseudomonas sp. Rits, which was phylogenetically related to Rhodopseudomonas (Rps.) palustris. In this study, the light-dependent and time-dependent ...
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Article
Composition and localization of bacteriochlorophyll a intermediates in the purple photosynthetic bacterium Rhodopseudomonas sp. Rits
Rhodopseudomonas sp. Rits is a recently isolated new species of photosynthetic bacteria and found to accumulate a significantly high amount of bacteriochlorophyll (BChl) a intermediates possessing non-, di- and t...
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Chapter and Conference Paper
Compositional Analysis of Carotenoids in a Purple Photosynthetic Bacteria, Rhodopseudomonas sp. Strain Rits, upon Irradiated Light Intensity
Recently We Isolated A Purple Photosynthetic Bacterium Rhodopseudomonas (Rps.) Sp. Strain Rits Which Is Phylogenetically Close To Rps. Palustris. Under Low-Light Conditions, This Strain Biosynthesized An Unusual ...
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Chapter and Conference Paper
Diversity and Localization of Bacteriochlorophylls Possessing Different 17-Propionate Groups in Purple Bacterial Antennae
In the final stage of biosynthesis of phytylated bacteriochlorophyll (BChl), the presence of BChl-a molecules possessing geranylgeranyl, dihydro- and tetrahydrogeranylgeranyl groups at the 17-propionate were repo...
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Article
Self-aggregation of synthetic zinc chlorophyll derivatives possessing multi-perfluoroalkyl chains in perfluorinated solvents
Zinc 31-hydroxy-131-oxo-chlorins possessing two, three, four and six perfluorooctyl chains were synthesized from naturally occurring chlorophyll-a. Only the synthetic zinc chlorin possessing six perfluorooctyl ch...
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Article
Stereochemical determination of chlorophyll-d molecule from Acaryochloris marina and its modification to a self-aggregative chlorophyll as a model of green photosynthetic bacterial antennae
Acaryochloris marina is a unique photosynthetic prokaryote containing chlorophyll(Chl)-d as a major photoactive pigment (over 95%). The molecular structure of Chl-d is proposed as the 3-formyl analog of Chl-a. Ho...
