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  1. No Access

    Article

    Characterization and function of wall-bound exo-β-glucanases of Lilium longiflorum pollen tubes

    Two exo-β-glucanases (LP-ExoI, 83 kDa and LP-ExoII, 71 kDa) were extracted and partially purified from the cell wall of Lilium longiflorum pollen tubes. Both LP-ExoI and LP-ExoII hydrolyzed laminarin (1,3-β-gluca...

    Toshihisa Kotake, Y.-Q. Li, Mai Takahashi, N. Sakurai in Sexual Plant Reproduction (2000)

  2. No Access

    Article

    Biosynthesis of pectic galactan by membrane-bound galactosyltransferase from soybean (Glycine max Merr.) seedlings

    We investigated the properties of a galactosyltransferase (GalT) that is involved in the synthesis of β-(1→4)-galactan side chains of pectins. A membrane preparation of etiolated 6-day-old soybean (Glycine max Me...

    Tomoyuki Konishi, Terukazu Mitome, Hiroyuki Hatsushika, Md. Ashraful Haque in Planta (2004)

  3. No Access

    Article

    Chain elongation of pectic β-(1→4)-galactan by a partially purified galactosyltransferase from soybean (Glycine max Merr.) hypocotyls

    Pectin is one of the major cell wall polysaccharides found in dicotyledonous plants. We have solubilized and partially purified a β-(1→4)-galactosyltransferase (GalT) involved in the synthesis of the β-(1→4)-g...

    Tomoyuki Konishi, Toshihisa Kotake, Yoichi Tsumuraya in Planta (2007)

  4. No Access

    Article

    Transient increase in the transcript levels of γ-tubulin complex genes during reorientation of cortical microtubules by gravity in azuki bean (Vigna angularis) epicotyls

    By hypergravity treatment, the percentage of cells with transverse microtubules was decreased, while that with longitudinal microtubules was increased in azuki bean (Vigna angularis) epicotyls. The expression of ...

    Kouichi Soga, Toshihisa Kotake, Kazuyuki Wakabayashi in Journal of Plant Research (2008)

  5. No Access

    Article

    Gummosis in grape hyacinth (Muscari armeniacum) bulbs: hormonal regulation and chemical composition of gums

    The purpose of this study was to investigate the hormonal regulation of gummosis in grape hyacinth (Muscari armeniacum) bulbs, focusing especially on the chemical composition of the gums. The application of ethep...

    Kensuke Miyamoto, Toshihisa Kotake, Makiko Sasamoto in Journal of Plant Research (2010)

  6. No Access

    Article

    Rice BRITTLE CULM 3 (BC3) encodes a classical dynamin OsDRP2B essential for proper secondary cell wall synthesis

    “Brittle culm” mutants found in Gramineae crops are suitable materials to study the mechanism of secondary cell wall formation. Through positional cloning, we have identified a gene responsible for the brittle...

    Ko Hirano, Toshihisa Kotake, Kumiko Kamihara, Kahori Tsuna, Tsutomu Aohara in Planta (2010)

  7. No Access

    Article

    Changes in the transcript levels of microtubule-associated protein MAP65-1 during reorientation of cortical microtubules in azuki bean epicotyls

    The relation between orientation of cortical microtubules and the transcript levels of a 65 kDa microtubule-associated protein (VaMAP65-1) was investigated along epicotyls of azuki bean (Vigna angularis) seedling...

    Kouichi Soga, Toshihisa Kotake, Kazuyuki Wakabayashi in Acta Physiologiae Plantarum (2012)

  8. No Access

    Article

    The role of extracellular polysaccharides produced by the terrestrial cyanobacterium Nostoc sp. strain HK-01 in NaCl tolerance

    The terrestrial cyanobacterium Nostoc sp. HK-01 was more tolerant to NaCl stress than the aquatic cyanobacterium Anabaena sp. PCC 7120 (also called Nostoc sp. PCC 7120) which is similar to Nostoc sp. HK-01 in phy...

    Hidehisa Yoshimura, Toshihisa Kotake, Tsutomu Aohara in Journal of Applied Phycology (2012)

  9. Article

    Open Access

    Biosynthesis of the carbohydrate moieties of arabinogalactan proteins by membrane-bound β-glucuronosyltransferases from radish primary roots

    A membrane fraction from etiolated 6-day-old primary radish roots (Raphanus sativus L. var hortensis) contained β-glucuronosyltransferases (GlcATs) involved in the synthesis of the carbohydrate moieties of arabin...

    Maya Endo, Toshihisa Kotake, Yoko Watanabe, Kazumasa Kimura, Yoichi Tsumuraya in Planta (2013)

  10. Article

    Open Access

    Precise estimation of genomic regions controlling lodging resistance using a set of reciprocal chromosome segment substitution lines in rice

    Severe lodging has occurred in many improved rice varieties after the recent strong typhoons in East and Southeast Asian countries. The indica variety Takanari possesses strong culm characteristics due to its lar...

    Taiichiro Ookawa, Ryo Aoba, Toshio Yamamoto, Tadamasa Ueda in Scientific Reports (2016)

  11. Article

    Open Access

    Metabolism of l-arabinose in plants

    l-Arabinose (l-Ara) is a plant-specific sugar accounting for 5–10 % of cell wall saccharides in Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa). l-Ara occurs in pectic arabinan, rhamno...

    Toshihisa Kotake, Yukiko Yamanashi, Chiemi Imaizumi in Journal of Plant Research (2016)

  12. No Access

    Article

    Screening of rice mutants with improved saccharification efficiency results in the identification of CONSTITUTIVE PHOTOMORPHOGENIC 1 and GOLD HULL AND INTERNODE 1

    The screening of rice mutants with improved cellulose to glucose saccharification efficiency (SE) identifies reduced xylan and/or ferulic acid, and a qualitative change of lignin to impact SE. ...

    Ko Hirano, Reiko Masuda, Wakana Takase, Yoichi Morinaka, Mayuko Kawamura in Planta (2017)

  13. Article

    Open Access

    Correction to: Metabolism of l-arabinose in plants

    The article “Metabolism of l-arabinose in plants”, written by “Toshihisa Kotake, Yukiko Yamanashi, Chiemi Imaizumi, Yoichi Tsumuraya”, was originally published Online First without open access. After publication ...

    Toshihisa Kotake, Yukiko Yamanashi, Chiemi Imaizumi in Journal of Plant Research (2018)

  14. No Access

    Article

    Degradative enzymes for type II arabinogalactan side chains in Bifidobacterium longum subsp. longum

    Type II arabinogalactan (AG) is a soluble prebiotic fiber stimulating the proliferation of bifidobacteria in the human gut. Larch AG, which is comprised of type II AG, is known to be utilized as an energy sour...

    Kiyotaka Fujita, Ayami Sakamoto, Satoshi Kaneko in Applied Microbiology and Biotechnology (2019)

  15. Article

    Open Access

    Wolfberry genomes and the evolution of Lycium (Solanaceae)

    Wolfberry Lycium, an economically important genus of the Solanaceae family, contains approximately 80 species and shows a fragmented distribution pattern among the Northern and Southern Hemispheres. Although seve...

    You-Long Cao, Yan-long Li, Yun-Fang Fan, Zhen Li, Kouki Yoshida in Communications Biology (2021)

  16. No Access

    Article

    Metabolomic analysis of rice brittle culm mutants reveals each mutant- specific metabolic pattern in each organ

    Plant cell walls play an important role in providing physical strength and defence against abiotic stress. Rice brittle culm (bc) mutants are a strength-decreased mutant because of abnormal cell walls, and it has...

    Atsuko Miyagi, Kazuhisa Mori, Toshiki Ishikawa, Satoshi Ohkubo in Metabolomics (2022)