5 Result(s)
within
Thomas P. Smith
Life Sciences
English
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Article
Open AccessHigh-throughput characterization of bacterial responses to complex mixtures of chemical pollutants
Our understanding of how microbes respond to micropollutants, such as pesticides, is almost wholly based on single-species responses to individual chemicals. However, in natural environments, microbes experien...
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Article
Novel NanoLuc substrates enable bright two-population bioluminescence imaging in animals
Sensitive detection of two biological events in vivo has long been a goal in bioluminescence imaging. Antares, a fusion of the luciferase NanoLuc to the orange fluorescent protein CyOFP, has emerged as a brigh...
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Article
Open AccessHorizontal gene transfer in bdelloid rotifers is ancient, ongoing and more frequent in species from desiccating habitats
Although prevalent in prokaryotes, horizontal gene transfer (HGT) is rarer in multicellular eukaryotes. Bdelloid rotifers are microscopic animals that contain a higher proportion of horizontally transferred, n...
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Article
Immunofluorescence localization of the epidermolytic toxin target in mouse epidermal cells and tissue
An epidermolytic toxin target was observed in keratohyalin granules of sectioned epidermis by a ‘direct’ fluorescence procedure using FTC-toxin, but not by an ‘indirect’ procedure using sequential reaction wit...
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Article
The binding of epidermolytic toxin fromStaphylococcus aureus to mouse epidermal tissue
Fluorescein-labelled epidermolytic toxin (FTC-toxin) ofStaphylococcus aureus and ferritin—toxin conjugate have been prepared and purified. FTC-toxin bound selectively to cryostat and resin-impregnated sections of...
