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Article
HT-smFISH: a cost-effective and flexible workflow for high-throughput single-molecule RNA imaging
The ability to visualize RNA in its native subcellular environment by using single-molecule fluorescence in situ hybridization (smFISH) has reshaped our understanding of gene expression and cellular functions....
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Article
Open AccessStochastic pausing at latent HIV-1 promoters generates transcriptional bursting
Promoter-proximal pausing of RNA polymerase II is a key process regulating gene expression. In latent HIV-1 cells, it prevents viral transcription and is essential for latency maintenance, while in acutely inf...
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Article
Open AccessA choreography of centrosomal mRNAs reveals a conserved localization mechanism involving active polysome transport
Local translation allows for a spatial control of gene expression. Here, we use high-throughput smFISH to screen centrosomal protein-coding genes, and we describe 8 human mRNAs accumulating at centrosomes. The...
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Article
Open AccessDistinct polymer physics principles govern chromatin dynamics in mouse and Drosophila topological domains
In higher eukaryotes, the genome is partitioned into large "Topologically Associating Domains" (TADs) in which the chromatin displays favoured long-range contacts. While a crumpled/fractal globule organization...
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Protocol
Analyzing the Dynamics of DNA Replication in Mammalian Cells Using DNA Combing
How cells duplicate their chromosomes is a key determinant of cell identity and genome stability. DNA replication can initiate from more than 100,000 sites distributed along mammalian chromosomes, yet a given ...
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Article
Open AccessComparison of different extraction techniques to profile microRNAs from human sera and peripheral blood mononuclear cells
microRNAs (miRNAs) play crucial roles in major biological processes and their deregulations are often associated with human malignancies. As such, they represent appealing candidates as targets of innovative t...
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Article
CBC–ARS2 stimulates 3′-end maturation of multiple RNA families and favors cap-proximal processing
The nuclear cap–binding complex (CBC) stimulates RNA maturation, but the mechanistic basis is not well understood. In vitro reconstitution experiments combined with functional analyses have revealed a new CBC com...
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Article
Open AccessModulated contact frequencies at gene-rich loci support a statistical helix model for mammalian chromatin organization
Despite its critical role for mammalian gene regulation, the basic structural landscape of chromatin in living cells remains largely unknown within chromosomal territories below the megabase scale.
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Protocol
Use of DNA Combing for Studying DNA Replication In Vivo in Yeast and Mammalian Cells
Plasticity is an inherent feature of chromosomal DNA replication in eukaryotes. Potential origins of DNA replication are made in excess, but are used (fired) in a partly stochastic, partly programmed manner th...
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Article
Rapid Determination of Protein Solubility and Stability Conditions for NMR Studies Using Incomplete Factorial Design
Sample preparation constitutes a crucial and limiting step in structural studies of proteins by NMR. The determination of the solubility and stability (SAS) conditions of biomolecules at millimolar concentrati...
