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Chapter
BioPBS™ (Polybutylene Succinate)
PBS (polybutylene succinate) is a biodegradable polymer that is spontaneously degraded into water and carbon dioxide due to the power of microorganisms under soil in the natural world. PBS has high heat resist...
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Open AccessImprovement of the affinity of an anti-rat P2X4 receptor antibody by introducing electrostatic interactions
We have recently developed a mouse monoclonal antibody (12–10H) binding to the head domain region in rat P2X4 receptor (rP2X4R, which is crucial for the pathogenesis of neuropathic pain) expressed on the cell ...
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Article
Production of scFv, Fab, and IgG of CR3022 Antibodies Against SARS-CoV-2 Using Silkworm-Baculovirus Expression System
COVID-19, caused by SARS-CoV-2, is currently spreading around the world and causing many casualties. Antibodies against such emerging infectious diseases are one of the important tools for basic viral research...
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Article
Open AccessA comprehensive analysis of novel disulfide bond introduction site into the constant domain of human Fab
Generally, intermolecular disulfide bond contribute to the conformational protein stability. To identify sites where intermolecular disulfide bond can be introduced into the Fab’s constant domain of the therap...
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Article
Evidence for detection of rat P2X4 receptor expressed on cells by generating monoclonal antibodies recognizing the native structure
P2X purinergic receptors are ATP-driven ionic channels expressed as trimers and showing various functions. A subtype, the P2X4 receptor present on microglial cells is highly involved in neuropathic pain. In th...
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Article
Selective and reversible modification of kinase cysteines with chlorofluoroacetamides
Irreversible inhibition of disease-associated proteins with small molecules is a powerful approach for achieving increased and sustained pharmacological potency. Here, we introduce α-chlorofluoroacetamide (CFA...
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Article
Open AccessIntracellular toxic advanced glycation end-products in cardiomyocytes may cause cardiovascular disease
Cardiovascular disease (CVD) is a lifestyle-related disease (LSRD) and one of the largest public health issues. Risk factors for CVD correlate with an excessive intake of glucose and/or fructose, which has bee...
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Article
Role of the osmolyte taurine on the folding of a model protein, hen egg white lysozyme, under a crowding condition
Taurine is one of the osmolytes that maintain the structure of proteins in cells exposed to denaturing environmental stressors. Recently, cryoelectron tomographic analysis of eukaryotic cells has revealed that...
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Article
Effect of the Conformational Stability of the CH2 Domain on the Aggregation and Peptide Cleavage of a Humanized IgG
To examine the effect of the conformational stability of the CH2 domain on aggregation and peptide cleavage of a humanized IgG1, we carried out size exclusion chromatography and sodium dodecyl sulfate polyacry...
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Article
Assignment of 1H, 13C and 15N resonances of N-terminal domain of DnaA protein
DnaA protein is a key protein in the initiation of chromosomal replication in Escherichia coli. We reported the assignments of 1H, 13C, and 15N resonances of N-terminal domain of Dna A (1–108) which contains the ...
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Article
Spontaneous Esophageal Perforation Related to a Duodenal Ulcer with Pyloric Stenosis: Report of a Case
This report describes a case of spontaneous esophageal perforation that was considered to be etiologically related to a duodenal ulcer with pyloric stenosis. The patient was a 54-year-old Japanese man who pre...
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Article
Solitary Thyroid Metastasis of Renal Clear Cell Carcinoma: Report of a Case
A case of solitary thyroid metastasis of renal clear cell carcinoma is described. The patient was a 77-year-old Japanese woman, who was referred to our department after a thyroid tumor was identified in May 1...
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Article
Letter to the Editor: Assignment of 1H and 15N resonances of mouse lysozyme M
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Article
Repeated Percutaneous Aspiration Therapy Prior to Surgery for a Pancreatic Pseudocyst: Report of a Case
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Article
Lymphangioma of the jejunum and mesentery presenting with acute abdomen in an adult
We describe a patient, 54-year-old woman, with lymphangiomas of the jejunum and mesentery presenting as acute abdomen. She had sudden onset of severe abdominal pain with guarding and fever. Physical examinati...
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Article
Mucinous cystadenoma of appendix concomitant with perityphlic granuloma
We describe a case of mucinous cystadenoma of the appendix with perityphlic granuloma. The patient developed a hard palpable mass in the right lower quadrant and barium enema inducted irregular bowel wall at t...
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Article
Evaluation of portal circulation through the superior mesenteric vein with an enteric capsule of [123I]iodoamphetamine
We report a method by which the contribution of the superior mesenteric vein to the portal blood flow can be evaluated noninvasively. An enteric-coated capsule containing [123I]iodoamphetamine is given by mouth 3...
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Article
Use of immobilized histidine in assay for endotoxin in patients with liver disease
TheLimulus amebocyte lysate (LAL) test has the disadvantage of being influenced by various inhibitors and activators. We have developed a method for the LAL reaction that involves the specific adsorption and isol...
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Article
Hypereosinophilic syndrome appearing as a focal defect on liver scan
The case reported here is of a 34-year-old man diagnosed as having hypereosinophilic syndrome. Blood tests showed a large increase in the number of eosinophils and a slight increase in asparate aminotransferas...
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Article
Diagnosis of hepatocellular carcinoma by factor analysis
We examined the usefulness of factor analysis for the diagnosis of hepatocellular carcinoma by analysis of the data obtained by radionuclide angiography. The data could be separated into two factors, a hepatic...
