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Article
Single-cell RNA sequencing provides a high-resolution roadmap for understanding the multicellular compartmentation of specialized metabolism
Monoterpenoid indole alkaloids (MIAs) are among the most diverse specialized metabolites in plants and are of great pharmaceutical importance. We leveraged single-cell transcriptomics to explore the spatial or...
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Article
Open AccessModeling human HSV infection via a vascularized immune-competent skin-on-chip platform
Herpes simplex virus (HSV) naturally infects skin and mucosal surfaces, causing lifelong recurrent disease worldwide, with no cure or vaccine. Biomimetic human tissue and organ platforms provide attractive alt...
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Article
Open AccessIntegrated DNA and RNA sequencing reveals early drivers involved in metastasis of gastric cancer
Gastric cancer (GC) is the second cause of cancer-related death and metastasis is an important cause of death. Considering difficulties in searching for metastatic driver mutations, we tried a novel strategy h...
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Article
Open AccessHybrid sequencing of the Gynostemma pentaphyllum transcriptome provides new insights into gypenoside biosynthesis
Gypenosides are a group of triterpene saponins from Gynostemma pentaphyllum that are the same as or very similar to ginsenosides from the Panax species. Several enzymes involved in ginsenoside biosynthesis have b...
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Article
Open AccessIso-Seq analysis of the Taxus cuspidata transcriptome reveals the complexity of Taxol biosynthesis
Taxus cuspidata is well known worldwide for its ability to produce Taxol, one of the top-selling natural anticancer drugs. However, current Taxol production cannot match the increasing needs of the market, and no...
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Article
Simulation of the osmosis-based drug encapsulation in erythrocytes
Drug-loaded erythrocytes have been proposed for the treatment of disease. A common way to load drugs into erythrocytes is to apply osmotic shock. Currently, osmosis-based drug encapsulation is studied mainly e...
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Article
A multistage-dialysis microdevice for extraction of cryoprotectants
In this study, we present a multistage-dialysis microdevice (MDM) for extraction of cryoprotectants (CPAs) from a CPA-laden cell suspension. We confirmed the functions of the key designs of the MDM using a flu...
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Article
Unloading of cryoprotectants from cryoprotectant-loaded cells on a microfluidic platform
In this paper, a multistep dilution-filtration microdevice (MDFD) is developed for unloading cryoprotectants from cryoprotectant-loaded cells. The MDFD contained a diluent producing region, a dilution-filtrati...
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Article
Open AccessTopical and Targeted Delivery of siRNAs to Melanoma Cells Using a Fusion Peptide Carrier
Topical application of siRNAs through the skin is a potentially effective strategy for the treatment of melanoma tumors. In this study, we designed a new and safe fusion peptide carrier SPACE-EGF to improve th...
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Article
Open AccessTheoretical optimization of the removal of cryoprotective agents using a dilution-filtration system
In the cryopreservation of blood, removing cryoprotectants from the cryopreserved blood safely and effectively is always being focused on. In our previous work, a dilution-filtration system was proposed to ach...
