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Article
Open AccessMature human induced pluripotent stem cell-derived cardiomyocytes promote angiogenesis through alpha-B crystallin
Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) can be used to treat heart diseases; however, the optimal maturity of hiPSC-CMs for effective regenerative medicine remains unclear. We ai...
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Chapter
Clinical Application of iPSC-Derived Cardiomyocytes in Patients with Advanced Heart Failure
Restoration of damaged cardiac tissues via supplementation with functional cardiomyocytes is an ideal medical treatment for advanced heart failure. Because human induced pluripotent stem cells (hiPSCs) can the...
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Article
Open AccessMetabolism of human pluripotent stem cells and differentiated cells for regenerative therapy: a focus on cardiomyocytes
Pluripotent stem cells (PSCs) exhibit promising application in regenerative therapy, drug discovery, and disease modeling. While several protocols for differentiating somatic cells from PSCs exist, their use i...
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Protocol
A Method for Cardiac Differentiation, Purification, and Cardiac Spheroid Production of Human Induced Pluripotent Stem Cells
Human induced pluripotent stem cells (hiPSCs) are one of the most promising cell sources for regenerative medicine. To realize the promise of hiPSCs for cardiac regenerative therapy, three major obstacles must...
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Article
Open AccessIncreased predominance of the matured ventricular subtype in embryonic stem cell-derived cardiomyocytes in vivo
Accumulating evidence suggests that human pluripotent stem cell-derived cardiomyocytes can affect “heart regeneration”, replacing injured cardiac scar tissue with concomitant electrical integration. However, e...
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Article
Open AccessToward the realization of cardiac regenerative medicine using pluripotent stem cells
Heart transplantation (HT) is the only radical treatment available for patients with end-stage heart failure that is refractory to optimal medical treatment and device therapies. However, HT as a therapeutic o...
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Article
Open AccessAn effective detachment system for human induced pluripotent stem cells cultured on multilayered cultivation substrates using resonance vibrations
Clinical application of human induced pluripotent stem cells (hiPSCs) has been hampered by the lack of a practical, scalable culture system. Stacked culture plates (SCPs) have recently attracted attention. How...
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Article
Open AccessEmerin plays a crucial role in nuclear invagination and in the nuclear calcium transient
Alteration of the nuclear Ca2+ transient is an early event in cardiac remodeling. Regulation of the nuclear Ca2+ transient is partly independent of the cytosolic Ca2+ transient in cardiomyocytes. One nuclear memb...
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Article
Open AccessManipulation of Pluripotent Stem Cell Metabolism for Clinical Application
Pluripotent stem cells (PSCs) have the capacity to differentiate into various types of cells, and are promising cell sources for regenerative therapy and drug screening. However, to realize the clinical applic...
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Article
Open AccessCryoinjury-induced acute myocardial infarction model and ameroid constrictor-induced ischemic heart disease model in adult micro-mini pigs for preclinical studies
Coronary artery diseases (CAD) are the most commonly occurring disorders in the developed countries. Development of new therapeutics and their success in clinical studies require confirmation of therapeutic ef...
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Chapter
Purification of Pluripotent Stem Cell-Derived Cardiomyocytes for Safe Cardiac Regeneration
Human pluripotent stem cells (PSCs), including embryonic stem cells (ESCs) and induced PSCs (iPSCs), have the potential to differentiate into various cells types and may be used as cell sources for regenerativ...
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Chapter
Road to Heart Regeneration with Induced Pluripotent Stem Cells
Restoration of cardiac function is the most desirable treatment of severe heart failure. However, effective regenerative therapy for the lost cardiomyocytes is currently an unmet medical need. Induced pluripot...
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Article
Open AccessANGPTL2 activity in cardiac pathologies accelerates heart failure by perturbing cardiac function and energy metabolism
A cardioprotective response that alters ventricular contractility or promotes cardiomyocyte enlargement occurs with increased workload in conditions such as hypertension. When that response is excessive, patho...
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Protocol
Patient-Specific Induced Pluripotent Stem Cell Models: Characterization of iPS Cell-Derived Cardiomyocytes
Despite significant advances in medical treatment, cardiovascular disease is still a major cause of morbidity and mortality in advanced countries. To improve the outcome, the further promotion of basic cardiov...
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Chapter
Future Treatment of Heart Failure Using Human iPSC-Derived Cardiomyocytes
Heart transplantation can drastically improve survival in patients with a failing heart; however, the shortage of donor hearts remains a serious problem with this treatment strategy and the successful clinical...
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Article
Erratum: G-CSF supports long-term muscle regeneration in mouse models of muscular dystrophy
Nature Communications 6: Article number: 6745 (2015); Published 13 April 2015; Updated 18 June 2015. The immunofluorescence images in Fig. 1b of the PDF version of this Article were inadvertently duplicated fr...
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Article
G-CSF supports long-term muscle regeneration in mouse models of muscular dystrophy
Duchenne muscular dystrophy (DMD) is a chronic and life-threatening disease that is initially supported by muscle regeneration but eventually shows satellite cell exhaustion and muscular dysfunction. The life-...
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Article
Nongenetic method for purifying stem cell–derived cardiomyocytes
Staining with a mitochondrial dye permits high-purity isolation of cardiomyocytes from embryonic and induced pluripotent stem cells of several species, without genetic modification.
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Article
Open AccessPeriodontal disease and atherosclerosis from the viewpoint of the relationship between community periodontal index of treatment needs and brachial-ankle pulse wave velocity
It has been suggested that periodontal disease may be an independent risk factor for the development of atherosclerosis. However, the relationship between periodontal disease and atherosclerosis has not been f...
