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  1. No Access

    Article

    Identification of genes that affect sensitivity to 5-bromodeoxyuridine in the yeast Saccharomyces cerevisiae

    Small molecules that exhibit biological effects have been successfully used to study various biological phenomena. 5-Bromodeoxyuridine (BrdU) is a thymidine analog that affects various biological processes, su...

    Michihiko Fujii, Kensuke Miki, Shinichi Takayama… in Molecular Genetics and Genomics (2010)

  2. No Access

    Article

    Overexpression of HAM1 gene detoxifies 5-bromodeoxyuridine in the yeast Saccharomyces cerevisiae

    5-Bromodeoxyuridine (BrdU) is known to modulate expression of particular genes, and eventually arrest cell division in mammalian and yeast cells. To study a molecular basis for these phenomena, we adopted a ge...

    Shinichi Takayama, Michihiko Fujii, Aya Kurosawa, Noritaka Adachi… in Current Genetics (2007)

  3. No Access

    Article

    Structural analysis of BAG1 cochaperone and its interactions with Hsc70 heat shock protein

    BAG-family proteins share a conserved protein interaction region, called the 'BAG domain', which binds and regulates Hsp70/Hsc70 molecular chaperones. This family of cochaperones functionally regulates signal ...

    Klára Briknarová, Shinichi Takayama, Lars Brive… in Nature Structural Biology (2001)

  4. No Access

    Article

    Co-expression of bcl-2 and bag-1, apoptosis suppressing genes, prolonged viable culture period of hybridoma and enhanced antibody production

    Human bcl-2 and bag-1 DNA were introduced into mouse hybridoma 2E3- O cells and expressed. The expression of bcl-2 in BCMGneo-bcl2 transfectants was confirmed by ELISA and that of bag-1 in pZeo-bag1 was confir...

    Satoshi Terada, Tomoaki Komatsu, Tetsuo Fujita, Ain Terakawa… in Cytotechnology (1999)

  5. No Access

    Article

    Erratum: Establishing apoptosis resistant cell lines for improving protein productivity of cell culture

    Cytotechnology 23 : 55–59, 1997.

    Eiji Suzuki, Satoshi Terada, Hiroshi Ueda, Tetsuo Fujita, Tomoaki Komatsu… in Cytotechnology (1998)

  6. No Access

    Article

    Anti-apoptotic genes, bag-1 and bcl-2, enabled hybridoma cells to survive under treatment for arresting cell cycle

    Hybridoma 2E3-O cells were transfected with bcl-2 alone or with bcl-2 and bag-1 in combination. The bcl-2/bag-1 transfectant survived maintaining viability above 75% for almost 5 days when the cells were treat...

    Satoshi Terada, Katsuyuki Fukuoka, Tetsuo Fujita, Tomoaki Komatsu… in Cytotechnology (1997)

  7. No Access

    Article

    Establishing apoptosis resistant cell lines for improving protein productivity of cell culture

    The authors established apoptosis resistant COS–1, myeloma, hybridoma, and Friend leukemia cell lines by genetically engineering cells, aiming at more efficient protein production by cell culture. COS–1 cells,...

    Eiji Suzuki, Satoshi Terada, Hiroshi Ueda, Tetsuo Fujita, Tomoaki Komatsu… in Cytotechnology (1997)