-
Article
Open AccessFunction of human pluripotent stem cell-derived photoreceptor progenitors in blind mice
Photoreceptor degeneration due to retinitis pigmentosa (RP) is a primary cause of inherited retinal blindness. Photoreceptor cell-replacement may hold the potential for repair in a completely degenerate retina...
-
Chapter
Generation of Hemangioblasts from Human Pluripotent Stem Cells
Hemangioblasts are progenitors with the capacity to differentiate into hematopoietic, endothelial, smooth muscle, and mesenchymal stromal cells and represent an excellent candidate of cell therapy for a variet...
-
Article
Open AccessPlatelets generated from human embryonic stem cells are functional in vitro and in the microcirculation of living mice
Platelets play an essential role in hemostasis and atherothrombosis. Owing to their short storage time, there is constant demand for this life-saving blood component. In this study, we report that it is feasib...
-
Protocol
Directed Differentiation of Red Blood Cells from Human Embryonic Stem Cells
Human embryonic stem cells (hESC) represent a new source of stem cells that can be propagated and expanded in vitro indefinitely, providing a potentially inexhaustible and donorless source of cells for human t...
-
Article
Open AccessGeneChip analysis of human embryonic stem cell differentiation into hemangioblasts: an in silicodissection of mixed phenotypes
Microarrays are being used to understand human embryonic stem cell (hESC) differentiation. Most differentiation protocols use a multi-stage approach that induces commitment along a particular lineage. Therefor...
-
Article
Derivation of human embryonic stem cells from single blastomeres
This protocol details a method to derive human embryonic stem (hES) cells from single blastomeres. Blastomeres are removed from morula (eight-cell)-stage embryos and cultured until they form multicell aggregat...
-
Article
Generation of functional hemangioblasts from human embryonic stem cells
Recent evidence suggests the existence of progenitor cells in adult tissues that are capable of differentiating into vascular structures as well as into all hematopoietic cell lineages. Here we describe an eff...
-
Article
Erratum: Human embryonic stem cell lines derived from single blastomeres
Nature 444, 481–485 (2006); doi:10.1038/nature05142 and Nature 444, 512 (2006) The last sentence of the penultimate paragraph of this Letter should read “Notably, individual morula (8–16 cell)-stage blastomere...
-
Article
Human embryonic stem cell lines derived from single blastomeres
-
Article
Human embryonic stem cell lines derived from single blastomeres
First published online on 23 August, the paper in this issue by Klimanskaya et al. reports a major advance: the generation of human stem cells from a single cell recovered during preimplantation genetic diagnosis...
-
Article
Embryonic and extraembryonic stem cell lines derived from single mouse blastomeres
The usual methods for establishing human embryonic stem cell lines are controversial as embryos are destroyed in the process. Two papers in this issue present different means of deriving such cells from mice w...
-
Article
Lineage-specific Mechanism of Drug and Radiation Resistance in Melanoma Mediated by Tyrosinase-related Protein 2
A major obstacle in the clinical management of malignant melanoma is its intrinsic resistance to chemotherapy and radiation therapy. Consequently, most patients with melanoma often do not respond to convention...
-
Article
Tyrosinase-related protein 2 as a mediator of melanoma specific resistance to cis-diamminedichloroplatinum(II): therapeutic implications
A major obstacle in the systemic treatment of advanced malignant melanoma is its intrinsic resistance to conventionally used chemotherapeutic agents. In order to investigate the mechanisms of this intrinsic re...
-
Chapter
Reactive Metabolites from N-Nitrosamines
N-Nitrosodialkylamines are metabolically activated by hydroxylation at the carbon atom alpha to the N-nitroso group. Spontaneous cleavage of the C-N bond in the α-hydroxynitrosamine leads to production of an alky...
