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Article
Open AccessThe Musashi proteins direct post-transcriptional control of protein expression and alternate exon splicing in vertebrate photoreceptors
The Musashi proteins, MSI1 and MSI2, are conserved RNA binding proteins with a role in the maintenance and renewal of stem cells. Contrasting with this role, terminally differentiated photoreceptor cells expre...
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Article
Open AccessDifferential reprogramming of breast cancer subtypes in 3D cultures and implications for sensitivity to targeted therapy
Screening for effective candidate drugs for breast cancer has shifted from two-dimensional (2D) to three-dimensional (3D) cultures. Here we systematically compared the transcriptomes of these different culture...
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Article
Open AccessUncovering the signaling landscape controlling breast cancer cell migration identifies novel metastasis driver genes
Ttriple-negative breast cancer (TNBC) is an aggressive and highly metastatic breast cancer subtype. Enhanced TNBC cell motility is a prerequisite of TNBC cell dissemination. Here, we apply an imaging-based RNA...
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Article
Open AccessADP-Ribosylation Factor-Like 2 (ARL2) regulates cilia stability and development of outer segments in rod photoreceptor neurons
Photoreceptor cells are specialized neurons with a sensory cilium carrying an elaborate membrane structure, the outer segment (OS). Inherited mutations in genes involved in ciliogenesis frequently result in OS...
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Article
Correction: Corrigendum: Ubiquitination of hnRNPA1 by TRAF6 links chronic innate immune signaling with myelodysplasia
Nat. Immunol. 18, 236–245; published online 26 December 2016; corrected after print 23 January 2017 In the version of this article initially published online, the 16th author's surname was spelled incorrectly ...
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Article
Ubiquitination of hnRNPA1 by TRAF6 links chronic innate immune signaling with myelodysplasia
Starczynowski and colleagues show that overexpression of TRAF6 in HSCs induces ubiquitination of the RNA-binding protein hnRNPA1 and alternative splicing of Arhgap1, which accounts for the hematopoietic defect...
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Article
Open AccessIdentification of small molecule modulators of HIV-1 Tat and Rev protein accumulation
HIV-1 replication is critically dependent upon controlled processing of its RNA and the activities provided by its encoded regulatory factors Tat and Rev. A screen of small molecule modulators of RNA processin...
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Protocol
Analysis of Alternative Pre-RNA Splicing in the Mouse Retina Using a Fluorescent Reporter
In vivo alternative splicing is controlled in a tissue and cell type specific manner. Often individual cellular components of complex tissues will express different splicing programs. Thus, when studying splic...
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Protocol
Medium Throughput Analysis of Alternative Splicing by Fluorescently Labeled RT-PCR
Reverse transcription-PCR (RT-PCR) is a core technique for detecting and quantifying alternative pre-mRNA splicing. RT-PCR is multistep process involving RNA isolation, reverse transcription, and PCR that is o...
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Article
Open AccessHIV-1 RNA processing as therapeutic target: characterization of small molecule modulators of HIV-1 RNA processing and transport
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Chapter and Conference Paper
High-Throughput Screening for Small Molecule Modulators of FGFR2-IIIb Pre-mRNA Splicing
Pre-mRNA splicing is the process in which a ribonucleoprotein complex called the spliceosome removes introns from a primary transcript and ligates its exons together [1]. The spliced exons produce an mRNA cont...
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Article
The splicing regulator Rbfox1 (A2BP1) controls neuronal excitation in the mammalian brain
The Rbfox family of RNA binding proteins mediate alternative splicing. Douglas Black and colleagues show that targeted deletion of the mouse Rbfox1 gene in the CNS alters splice isoforms of a subset of genes and ...
