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Article
A protein assembly mediates **st localization and gene silencing
Nuclear compartments have diverse roles in regulating gene expression, yet the molecular forces and components that drive compartment formation remain largely unclear1. The long non-coding RNA **st establishes an...
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Article
Publisher Correction: A protein assembly mediates **st localization and gene silencing
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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Protocol
Methods for Extraction of RNA, Proteins, or Protein Complexes from Subcellular Compartments of Eukaryotic Cells
Eukaryotic organelles or subcellular compartments can be selectively isolated based on their physical density and their stability in the presence of nonionic detergents. This chapter describes a protocol for t...
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Article
The splicing regulator Rbfox1 (A2BP1) controls neuronal excitation in the mammalian brain
The Rbfox family of RNA binding proteins mediate alternative splicing. Douglas Black and colleagues show that targeted deletion of the mouse Rbfox1 gene in the CNS alters splice isoforms of a subset of genes and ...
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Article
Polypyrimidine tract binding protein controls the transition from exon definition to an intron defined spliceosome
The polypyrimidine tract binding protein (PTB) binds pre-mRNAs to alter splice-site choice. We characterized a series of spliceosomal complexes that assemble on a pre-mRNA under conditions of either PTB-mediat...
