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Article
Open AccessEffect of hormone-induced plasma membrane phosphatidylinositol 4,5-bisphosphate depletion on receptor endocytosis suggests the importance of local regulation in phosphoinositide signaling
Phosphatidylinositol 4,5-bisphosphate (PIP2) has been shown to be critical for the endocytosis of G protein-coupled receptors (GPCRs). We have previously demonstrated that depletion of PIP2 by chemically induc...
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Article
Open AccessCapture at the ER-mitochondrial contacts licenses IP3 receptors to stimulate local Ca2+ transfer and oxidative metabolism
Endoplasmic reticulum-mitochondria contacts (ERMCs) are restructured in response to changes in cell state. While this restructuring has been implicated as a cause or consequence of pathology in numerous system...
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Article
Open AccessPalmitoylation targets the calcineurin phosphatase to the phosphatidylinositol 4-kinase complex at the plasma membrane
Calcineurin, the conserved protein phosphatase and target of immunosuppressants, is a critical mediator of Ca2+ signaling. Here, to discover calcineurin-regulated processes we examined an understudied isoform, CN...
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Protocol
Development of Nonspecific BRET-Based Biosensors to Monitor Plasma Membrane Inositol Lipids in Living Cells
There are several difficulties to face when investigating the role of phosphoinositides. Although they are present in most organelles, their concentration is very low, sometimes undetectable with the available...
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Article
Open AccessTrans-mitochondrial coordination of cristae at regulated membrane junctions
Reminiscent of bacterial quorum sensing, mammalian mitochondria participate in inter-organelle communication. However, physical structures that enhance or enable interactions between mitochondria have not been...
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Article
Open AccessInvestigation of the PtdIns(4,5)P2 dependence of plasma membrane receptor endocytosis in living cells
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Article
Open AccessTransactivation within the AT1 angiotensin receptor homodimer: the role of the conserved DRY motif
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Article
Visualization and manipulation of phosphoinositide dynamics in live cells using engineered protein domains
There is hardly a membrane-associated molecular event that is not regulated by phosphoinositides, a minor but critically important class of phospholipids of cellular membranes. The rapid formation, elimination...
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Article
The MUMO (minimal under-restraining minimal over-restraining) method for the determination of native state ensembles of proteins
While reliable procedures for determining the conformations of proteins are available, methods for generating ensembles of structures that also reflect their flexibility are much less well established. Here we...
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Chapter
Modeling DNA Deformation
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Article
Quantum mechanical/molecular mechanical study of three stationary points along the deacylation step of the catalytic mechanism of elastase
A large amount of experimental as well as theoretical information is available about the mechanism of serine proteases, but many questions remain unanswered. Here we study the deacylation step of the reaction...
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Article
Electrophysiological study on the high K+ sensitivity of rat glomerulosa cells
Elevation of extracellular potassium concentration by as little as some tenth of mM activates rat adrenal glomerulosa cells. In the present study some factors responsible for this high K+ sensitivity were examin...
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Chapter
Correlation of Seismo- and Magnetostratigraphy in Southeastern Hungary
Correlation of results from magnetostratigraphic and seismic-reflection studies indicate that the Pannonian Basin, during the postrift phase of its evolution (middle Miocene to present), became filled by sedim...
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Chapter
Structural Control on Hydrocarbon Accumulation in the Pannonian Basin, Hungary
The Pannonian Basin is a back-arc basin superimposed on the Alpine compressional megasuture that resulted from continental collision between Europe and smaller continental fragments following southward subduct...
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Chapter
Structure of the Békés Basin Inferred from Seismic Reflection, Well and Gravity Data
The Békés basin (areal extent 3900 km2) is a northwest-trending, Neogene basin located in southeast Hungary. The basin contains over 6500 m of synrift and postrift sedimentary fill. Middle Miocene synrift deposit...
