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Protocol
Screening Glycosyltransferases for Polyphenol Modifications
Glycosyltransferases offer the opportunity to glycosylate a variety of substrates including health beneficial molecules like flavonoids in a regiospecific manner. Flavonoids are plant secondary metabolites tha...
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Protocol
Screening for Cellulase Encoding Clones in Metagenomic Libraries
For modern biotechnology there is a steady need to identify novel enzymes. In biotechnological applications, however, enzymes often must function under extreme and nonnatural conditions (i.e., in the presence ...
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Article
Open AccessDeep metagenome and metatranscriptome analyses of microbial communities affiliated with an industrial biogas fermenter, a cow rumen, and elephant feces reveal major differences in carbohydrate hydrolysis strategies
The diverse microbial communities in agricultural biogas fermenters are assumed to be well adapted for the anaerobic transformation of plant biomass to methane. Compared to natural systems, biogas reactors are...
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Article
Metagenomic cellulases highly tolerant towards the presence of ionic liquids—linking thermostability and halotolerance
Cellulose is an important renewable resource for the production of bioethanol and other valuable compounds. Several ionic liquids (ILs) have been described to dissolve water-insoluble cellulose and/or wood. Th...
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Protocol
Screening for Cellulases with Industrial Value and Their Use in Biomass Conversion
Cellulose is an easily renewable and highly occurring resource. To take advantage of this great potential, there is a constant need of new cellulose degrading enzymes. In industrial applications enzymes have t...
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Protocol
Screening for Cellulase-Encoding Clones in Metagenomic Libraries
Modern biotechnology has the steady need to continuously identify novel enzymes for use in biotechnological applications. In industrial applications, however, enzymes often have to function under extreme and n...
