Abstract
Modern biotechnology has the steady need to continuously identify novel enzymes for use in biotechnological applications. In industrial applications, however, enzymes often have to function under extreme and nonnatural conditions (i.e., in the presence of solvents, high temperature and/or at extreme pH values). Cellulases have many industrial applications from the generation of bioethanol, a realistic long-term energy source, to the finishing of textiles. These industrial processes require cellulolytic activity under a range of pH, temperature, and ionic conditions, and they are usually carried out by mixtures of cellulases. Investigation of the broad diversity of cellulolytic enzymes involved in the natural degradation of cellulose is necessary for optimization of these processes.
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References
Streit, W.R. and Schmitz, R.A. (2004) MetaÂgenomics – the key to the uncultured microbes. Curr Opin Microbiol 7, 492–498.
Daniel, R. (2004) The soil metagenome – a rich resource for the discovery of novel natural products. Curr Opin Biotechnol 15, 199–204.
Schmeisser, C., Steele, H., and Streit, W.R. (2007) Metagenomics, biotechnology with non-culturable microbes. Appl Microbiol Biotechnol 75, 955–962.
Schmidt, T.M., DeLong, E.F., and Pace, N.R. (1991) Analysis of a marine picoplankton commuÂnity by 16S rRNA gene cloning and sequencing. J Bacteriol 173, 4371–4378.
Ferrer, M., Golyshina, O.V., Chernikova, T.N., Khachane, A.N., Reyes-Duarte, D., Santos, V.A., et al. (2005) Novel hydrolase diversity retrieved from a metagenome library of bovine rumen microflora. Environ Microbiol 7, 1996–2010.
Ferrer, M., Golyshina, O.V., Plou, F.J., Timmis, K.N., and Golyshin, P.N. (2005) A novel alpha-glucosidase from the acidophilic archaeon Ferroplasma acidiphilum strain Y with high transglycosylation activity and an unusual catalytic nucleophile. Biochem J 391, 269–276.
Beloqui, A., Pita, M., Polaina, J., Martinez-Arias, A., Golyshina, O.V., Zumarraga, M., et al. (2006) Novel polyphenol oxidase mined from a metagenome expression library of bovine rumen: biochemical properties, structural analysis, and phylogenetic relationships. J Biol Chem 281, 22933–22942.
Voget, S., Leggewie, C., Uesbeck, A., Raasch, C., Jaeger, K.-E., and Streit, W.R. (2003) ProsÂpecting for novel biocatalysts in a soil metagenome. Appl Environ Microbiol 69, 6235–6242.
Voget, S., Steele, H.L., and Streit, W.R. (2006) Characterization of a metagenome-derived halotolerant cellulase. J Biotechnol 126, 26–36.
Grant, S., Sorokin, D.Y., Grant, W.D., Jones, B.E., and Heaphy, S. (2004) A phylogenetic analysis of Wadi el Natrun soda lake cellulase enrichment cultures and identification of cellulase genes from these cultures. Extremophiles 8, 421–429.
Rees, H.C., Grant, S., Jones, B., Grant, W.D., and Heaphy, S. (2003) Detecting cellulase and esterase enzyme activities encoded by novel genes present in environmental DNA libraries. Extremophiles 7, 415–421.
Healy, F.G., Ray, R.M., Aldrich, H.C., Wilkie, A.C., Ingram, L.O., and Shanmugam, K.T. (1995) Direct isolation of functional genes encoding cellulases from the microbial consortia in a thermophilic, anaerobic digester maintained on lignocellulose. Appl Microbiol Biotechnol 43, 667–674.
Feng, Y., Duan, C.J., Pang, H., Mo, X.C., Wu, C.F., Yu, Y., et al. (2007) Cloning and identification of novel cellulase genes from uncultured microorganisms in rabbit cecum and characterization of the expressed cellulases. Appl Microbiol Biotechnol 75, 319–328.
Pottkämper, J., Barthen, P., Ilmberger, N., Schwaneberg, U., Schenk, A., Schulte, M., et al. (2009) Applying metagenomics for the identification of bacterial cellulases that are stable in ionic liquids. Green Chem 11, 957–965.
Guo, H., Feng, Y., Mo, X., Duan, C., Tang, J., and Feng, J. (2008) Cloning and expression of a beta-glucosidase gene umcel3G from metaÂgenome of buffalo rumen and characterization of the translated product. Sheng Wu Gong Cheng Xue Bao 24, 232–238.
Pang, H., Zhang, P., Duan, C.J., Mo, X.C., Tang, J.L., and Feng, J.X. (2009) Identification of cellulase genes from the metagenomes of compost soils and functional characterization of one novel endoglucanase. Curr Microbiol 58, 404–408.
Warnecke, F., Luginbuhl, P., Ivanova, N., Ghassemian, M., Richardson, T.H., Stege, J.T., et al. (2007) Metagenomic and functional anaÂlysis of hindgut microbiota of a wood-feeding higher termite. Nature 450, 560–565.
Heinze, T., Schwikal, K., and Barthel, S. (2005) Ionic liquids as reaction medium in cellulose functionalization. Macromol Biosci 5, 520–525.
Swatloski, R.P., Spear, S.K., Holbrey, J.D., and Rogers, R.D. (2002) Dissolution of cellulose [correction of cellose] with ionic liquids. J Am Chem Soc 124, 4974–4975.
Wu, J., Zhang, J., Zhang, H., He, J., Ren, Q., and Guo, M. (2004) Homogeneous acetylation of cellulose in a new ionic liquid. Biomacromole-cules 5, 266–268.
Beguin, P. and Aubert, J.P. (1994) The bioloÂgical degradation of cellulose. FEMS Microbiol Rev 13, 25–58.
Birsan, C., Johnson, P., Joshi, M., MacLeod, A., McIntosh, L., Monem, V., et al. (1998) Mechanisms of cellulases and xylanases. Biochem Soc Trans 26, 156–160.
Hilden, L. and Johansson, G. (2004) Recent developments on cellulases and carbohydrate-binding modules with cellulose affinity. Biotechnol Lett 26, 1683–1693.
Bayer, E.A., Chanzy, H., Lamed, R., and Shoham, Y. (1998) Cellulose, cellulases and cellulosomes. Curr Opin Struct Biol 8, 548–557.
Kumar, R., Singh, S., and Singh, O.V. (2008) Bioconversion of lignocellulosic biomass: biochemical and molecular perspectives. J Ind Microbiol Biotechnol 35, 377–391.
Ando, S., Ishida, H., Kosugi, Y., and Ishikawa, K. (2002) Hyperthermostable endoglucanase from Pyrococcus horikoshii. Appl Environ Microbiol 68, 430–433.
Lynd, L.R. and Zhang, Y. (2002) Quantitative determination of cellulase concentration as distinct from cell concentration in studies of microbial cellulose utilization: analytical framework and methodological approach. Biotechnol Bioeng 77, 467–475.
Schwarz, W.H. (2001) The cellulosome and cellulose degradation by anaerobic bacteria. Appl Microbiol Biotechnol 56, 634–649.
Zhang, Y.H. and Lynd, L.R. (2004) Toward an aggregated understanding of enzymatic hydrolysis of cellulose: noncomplexed cellulase systems. Biotechnol Bioeng 88, 797–824.
Bolam, D.N., Ciruela, A., McQueen-Mason, S., Simpson, P., Williamson, M.P., Rixon, J.E., et al. (1998) Pseudomonas cellulose-binding domains mediate their effects by increasing enzyme substrate proximity. Biochem J 331(Pt 3), 775–781.
Carvalho, A.L., Goyal, A., Prates, J.A., Bolam, D.N., Gilbert, H.J., Pires, V.M., et al. (2004) The family 11 carbohydrate-binding module of Clostridium thermocellum Lic26A-Cel5E accommodates beta-1,4- and beta-1,3-1,4-mixed linked glucans at a single binding site.J Biol Chem 279, 34785–34793.
Coutinho, J.B., Gilkes, N.R., Kilburn, D.G., Warren, RA.J., and Miller, R.C., Jr. (1993) The nature of the cellulose-binding domain effects the activities of a bacterial endoglucanase on different forms of cellulose. FEMS Microbiol Lett 113, 211–217.
Fontes, C.M., Clarke, J.H., Hazlewood, G.P., Fernandes, T.H., Gilbert, H.J., and Ferreira, L.M. (1997) Possible roles for a non-modular, thermostable and proteinase-resistant cellulase from the mesophilic aerobic soil bacterium Cellvibrio mixtus. Appl MicrobiolBiotechnol 48, 473–479.
Cazemier, A.E., Verdoes, JC., Op den Camp, HJ., Hackstein, J.H., and van Ooyen, A.J. (1999) A beta-1,4-endoglucanase-encoding gene from Cellulomonas pachnodae. Appl Microbiol Biotechnol 52, 232–239.
Sanchez-Torres, J., Perez, P., and Santamaria, R.I. (1996) A cellulase gene from a new alkalophilic Bacillus sp. (strain N186-1). Its cloning, nucleotide sequence and expression in EscheriÂchia coli. Appl MicrobiolBiotechnol 46, 149–155.
Solingen, P., Meijer, D., Kleij, W., Barnett, C., Bolle, R., Power, S., et al. (2001) Cloning and expression of an endocellulase gene from a novel streptomycete isolated from an East African soda lake. Extremophiles 5, 333.
Handelsman, J., Rondon, M.R., Brady, S.F., Clardy, J., and Goodman, R.M. (1998) Molecular biological access to the chemistry of unknown soil microbes: a new frontier for natural products. Chem Biol 5, R245–R249.
Brosius, J., Ullrich, A., Raker, M.A., Gray, A., Dull, T.J., Gutell, R.R., et al. (1981) Construction and fine map** of recombinant plasmids containing the rrnB ribosomal RNA operon of E. coli. Plasmid 6, 112–118.
Kane, M.D., Poulsen, L.K., and Stahl, D.A. (1993) Monitoring the enrichment and isolation of sulfate-reducing bacteria by using oligonucleotide hybridization probes designed from environmentally derived 16S rRNA sequences. Appl Environ Microbiol 59, 682–686.
Teather, R.M. and Wood, P.J. (1982) Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl Environ Microbiol 43, 777–780.
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Ilmberger, N., Streit, W.R. (2010). Screening for Cellulase-Encoding Clones in Metagenomic Libraries. In: Streit, W., Daniel, R. (eds) Metagenomics. Methods in Molecular Biology, vol 668. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-823-2_12
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DOI: https://doi.org/10.1007/978-1-60761-823-2_12
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