Metagenomics
Methods and Protocols
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Open AccessCommunity dynamics and metagenomic analyses reveal Bacteroidota's role in widespread enzymatic Fucus vesiculosus cell wall degradation
Enzymatic degradation of algae cell wall carbohydrates by microorganisms is under increasing investigation as marine organic matter gains more value as a sustainable resource. The fate of carbon in the marine ...
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Open AccessNew dienelactone hydrolase from microalgae bacterial community-Antibiofilm activity against fish pathogens and potential applications for aquaculture
Biofilms are resistant to many traditional antibiotics, which has led to search for new antimicrobials from different and unique sources. To harness the potential of aquatic microbial resources, we analyzed th...
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Open AccessNovel marine metalloprotease—new approaches for inhibition of biofilm formation of Stenotrophomonas maltophilia
Many marine organisms produce bioactive molecules with unique characteristics to survive in their ecological niches. These enzymes can be applied in biotechnological processes and in the medical sector to repl...
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Open AccessAn archaeal lid-containing feruloyl esterase degrades polyethylene terephthalate
Polyethylene terephthalate (PET) is a commodity polymer known to globally contaminate marine and terrestrial environments. Today, around 80 bacterial and fungal PET-active enzymes (PETases) are known, originat...
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Screening Metagenomes for Algae Cell Wall Carbohydrates Degrading Hydrolases in Enrichment Cultures
Sustainable use of is one of the key challenges for the future. An increasing focus is on marine organic matter, mostly . New biotechnological tools for processing high amounts of micro- and macroalgae are ...
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The PET-Degrading Potential of Global Metagenomes: From In Silico Mining to Active Enzymes
Against the background of the steadily increasing amount of plastic waste in the sea and on land, it is more important than ever to find ways out of this situation. In recent years, microorganisms have been di...
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Metagenomic Screening of a Novel PET Esterase via In Vitro Expression System
Metagenomic screening is a widely applied biotechnological approach for screening of novel industrial enzymes. The traditional method of metagenomic screening is based on the functional analyses of heterologou...
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Open AccessA novel metagenome-derived viral RNA polymerase and its application in a cell-free expression system for metagenome screening
The mining of genomes from non-cultivated microorganisms using metagenomics is a powerful tool to discover novel proteins and other valuable biomolecules. However, function-based metagenome searches are often ...
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Marine Mikroorganismen für den Plastikabbau
Millions of tons of plastic waste are released into the marine environment every year. While they steadily accumulate, synthetic polymers provide a habitat for microorganisms. This denominated Plastisphere has...
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Open AccessInterference and co-existence of staphylococci and Cutibacterium acnes within the healthy human skin microbiome
Human skin is populated by trillions of microbes collectively called the skin microbiome. Staphylococcus epidermidis and Cutibacterium acnes are among the most abundant members of this ecosystem, with described r...
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Open AccessMicroorganisms harbor keys to a circular bioeconomy making them useful tools in fighting plastic pollution and rising CO2 levels
The major global and man-made challenges of our time are the fossil fuel-driven climate change a global plastic pollution and rapidly emerging plant, human and animal infections. To meet the necessary global c...
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Open AccessComparison of three amplicon sequencing approaches to determine staphylococcal populations on human skin
Staphylococci are important members of the human skin microbiome. Many staphylococcal species and strains are commensals of the healthy skin microbiota, while few play essential roles in skin diseases such as ...
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Open AccessThe Komagataeibacter europaeus GqqA is the prototype of a novel bifunctional N-Acyl-homoserine lactone acylase with prephenate dehydratase activity
Previously, we reported the isolation of a quorum quenching protein (QQ), designated GqqA, from Komagataeibacter europaeus CECT 8546 that is highly homologous to prephenate dehydratases (PDT) (Valera et al. in Mi...
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Open AccessA promiscuous ancestral enzyme´s structure unveils protein variable regions of the highly diverse metallo-β-lactamase family
The metallo-β-lactamase fold is an ancient protein structure present in numerous enzyme families responsible for diverse biological processes. The crystal structure of the hyperthermostable crenarchaeal enzyme...
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Open AccessCysteine: an overlooked energy and carbon source
Biohybrids composed of microorganisms and nanoparticles have emerged as potential systems for bioenergy and high-value compound production from CO2 and light energy, yet the cellular and metabolic processes withi...
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Open AccessThe phylogenetic landscape and nosocomial spread of the multidrug-resistant opportunist Stenotrophomonas maltophilia
Recent studies portend a rising global spread and adaptation of human- or healthcare-associated pathogens. Here, we analyse an international collection of the emerging, multidrug-resistant, opportunistic patho...
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High-throughput amplicon sequencing demonstrates extensive diversity of xylanase genes in the sediment of soda lake Dabusu
To explore the diversity of glycoside hydrolase family 10 xylanase genes in the sediment of soda lake Dabusu by using high-throughput amplicon sequencing based on the Illumina HiSeq2500 platform.
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Open AccessThe Thaumarchaeon N. gargensis carries functional bioABD genes and has a promiscuous E. coli ΔbioH-complementing esterase EstN1
Biotin is an essential cofactor required for carboxylation and decarboxylation reactions in all domains of life. While biotin biosynthesis in most Bacteria and Eukarya is well studied, the complete pathway for...
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