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Open AccessAuthor Correction: Multiscale reorganization of the genome following DNA damage facilitates chromosome translocations via nuclear actin polymerization
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Open AccessMultiscale reorganization of the genome following DNA damage facilitates chromosome translocations via nuclear actin polymerization
Nuclear actin-based movements have been shown to orchestrate clustering of DNA double-strand breaks (DSBs) into homology-directed repair domains. Here we describe multiscale three-dimensional genome reorganiza...
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DksA and DNA double-strand break repair
We use genetic assays to suggest that transcription-coupled repair or new origin formation in Escherichia coli involves removal of RNAP to create an RNA primer for DNA synthesis. Transcription factor DksA was sho...
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Open AccessRNA Stabilizes Transcription-Dependent Chromatin Loops Induced By Nuclear Hormones
We show that transcription induced by nuclear receptors for estrogen (E2) or retinoic acid (RA) is associated with formation of chromatin loops that juxtapose the 5’ end (containing the promoter) with the enhance...
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Open AccessStructure and nucleic acid binding properties of KOW domains 4 and 6–7 of human transcription elongation factor DSIF
The human transcription elongation factor DSIF is highly conserved throughout all kingdoms of life and plays multiple roles during transcription. DSIF is a heterodimer, consisting of Spt4 and Spt5 that interac...
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Nuclear ARP2/3 drives DNA break clustering for homology-directed repair
DNA double-strand breaks repaired by non-homologous end joining display limited DNA end-processing and chromosomal mobility. By contrast, double-strand breaks undergoing homology-directed repair exhibit extens...
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Open AccessHigh-coverage methylation data of a gene model before and after DNA damage and homologous repair
Genome-wide methylation analysis is limited by its low coverage and the inability to detect single variants below 10%. Quantitative analysis provides accurate information on the extent of methylation of single...
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Open AccessDNA damage and Repair Modify DNA methylation and Chromatin Domain of the Targeted Locus: Mechanism of allele methylation polymorphism
We characterize the changes in chromatin structure, DNA methylation and transcription during and after homologous DNA repair (HR). We find that HR modifies the DNA methylation pattern of the repaired segment. ...
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Open AccessProteolysis of MOB1 by the ubiquitin ligase praja2 attenuates Hippo signalling and supports glioblastoma growth
Human glioblastoma is the most frequent and aggressive form of brain tumour in the adult population. Proteolytic turnover of tumour suppressors by the ubiquitin–proteasome system is a mechanism that tumour cel...
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Open AccessThe rate of the molecular clock and the cost of gratuitous protein synthesis
The nature of the protein molecular clock, the protein-specific rate of amino acid substitutions, is among the central questions of molecular evolution. Protein expression level is the dominant determinant of ...
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The nus mutations affect transcription termination in Escherichia coli
The nusA1 and nusB5 mutations result in a partial suppression of polarity and thus transcription termination in Escherichia coli. As these mutations block the transcription antitermination activity of bacteriopha...
