-
Article
Open AccessCellular identity and Ca2+ signaling activity of the non-reproductive GnRH system in the Ciona intestinalis type A (Ciona robusta) larva
Tunicate larvae have a non-reproductive gonadotropin-releasing hormone (GnRH) system with multiple ligands and receptor heterodimerization enabling complex regulation. In Ciona intestinalis type A larvae, one of ...
-
Article
Open AccessOrchestrated ensemble activities constitute a hippocampal memory engram
The brain stores and recalls memories through a set of neurons, termed engram cells. However, it is unclear how these cells are organized to constitute a corresponding memory trace. We established a unique ima...
-
Article
Open AccessWide and Deep Imaging of Neuronal Activities by a Wearable NeuroImager Reveals Premotor Activity in the Whole Motor Cortex
Wearable technologies for functional whole brain imaging in freely moving animals would advance our understanding of cognitive processing and adaptive behavior. Fluorescence imaging can visualize the activity ...
-
Article
Open AccessSuper-wide-field two-photon imaging with a micro-optical device moving in post-objective space
Wide-field imaging of neural activity at a cellular resolution is a current challenge in neuroscience. To address this issue, wide-field two-photon microscopy has been developed; however, the field size is lim...
-
Article
Open AccessRole of tyramine in calcium dynamics of GABAergic neurons and escape behavior in Caenorhabditis elegans
Tyramine, known as a “trace amine” in mammals, modulates a wide range of behavior in invertebrates; however, the underlying cellular and circuit mechanisms are not well understood. In the nematode Caenorhabditis ...
-
Article
Open AccessIn vivo wide-field calcium imaging of mouse thalamocortical synapses with an 8 K ultra-high-definition camera
In vivo wide-field imaging of neural activity with a high spatio-temporal resolution is a challenge in modern neuroscience. Although two-photon imaging is very powerful, high-speed imaging of the activity of indi...
-
Article
Allogeneic transplantation of iPS cell-derived cardiomyocytes regenerates primate hearts
Allogenic induced pluripotent stem cell-derived cardiomyocytes transplanted directly into infarcted cynomolgus monkey hearts show electrical coupling with host cardiomyocytes improve cardiac contractile functi...
-
Article
Open AccessThe integrative role of orexin/hypocretin neurons in nociceptive perception and analgesic regulation
The level of wakefulness is one of the major factors affecting nociception and pain. Stress-induced analgesia supports an animal’s survival via prompt defensive responses against predators or competitors. Prev...
-
Article
Olfactory receptor for prostaglandin F2α mediates male fish courtship behavior
In fish, prostaglandin F2α is a female hormone regulating ovulation, but it is also a pheromone that triggers male reproductive behavior. In this study, the authors identified an olfactory receptor for prostaglan...
-
Article
Open AccessCalcium imaging reveals glial involvement in transcranial direct current stimulation-induced plasticity in mouse brain
Transcranical direct current stimulation (tDCS) is a treatment known to ameliorate various neurological conditions and enhance memory and cognition in humans. tDCS has gained traction for its potential therape...
-
Article
Erratum: Rational design of a high-affinity, fast, red calcium indicator R-CaMP2
Nat. Methods 12, 64–70 (2015); published online 24 November 2014; corrected after print 11 June 2015 In the version of this article initially published, GenBank/EMBL/DDBJ accession codes for R-GECO2L and R-CaM...
-
Article
Rational design of a high-affinity, fast, red calcium indicator R-CaMP2
An improved genetically encoded red calcium sensor enables the monitoring of neuronal activity in cell culture and in vivo. R-CaMP2 has fast kinetics and a high affinity to calcium and can follow action potential...
-
Chapter
Optogenetic Manipulation and Probing
Controlling and monitoring the activities of defined cell populations should provide powerful methodologies for better understanding individual cellular functions in vivo. To enable the control and monitoring ...
-
Article
Reward-timing-dependent bidirectional modulation of cortical microcircuits during optical single-neuron operant conditioning
Animals rapidly adapt to environmental change. To reveal how cortical microcircuits are rapidly reorganized when an animal recognizes novel reward contingency, we conduct two-photon calcium imaging of layer 2/...
-
Article
Open AccessImaging intraorganellar Ca2+ at subcellular resolution using CEPIA
The endoplasmic reticulum (ER) and mitochondria accumulate Ca2+ within their lumens to regulate numerous cell functions. However, determining the dynamics of intraorganellar Ca2+ has proven to be difficult. Here ...
-
Protocol
Imaging of Astrocytic Activity in Living Rodents
Mounting evidence from in vitro experiments supports bidirectional interactions between astrocytes and neurons, suggesting glial involvement in neuronal information processing in the brain. Elevation of the cy...
-
Chapter and Conference Paper
Toward Living Tactile Sensors
The authors aim to realize a ”Cell Tactile Sensor” which is integrated tactile receptors built by cultured cells. We regard the cells cultured on the PDMS membrane as the force receptors. We here report on int...
-
Article
Open AccessLIS1-dependent retrograde translocation of excitatory synapses in develo** interneuron dendrites
Synaptic remodelling coordinated with dendritic growth is essential for proper development of neural connections. After establishment of synaptic contacts, synaptic junctions are thought to become stationary a...
-
Article
A high signal-to-noise Ca2+ probe composed of a single green fluorescent protein
Recently, several groups have developed green fluorescent protein (GFP)-based Ca2+ probes. When applied in cells, however, these probes are difficult to use because of a low signal-to-noise ratio. Here we report ...
