-
Article
Open AccessAuthor Correction: Mechanical stimulation of induced pluripotent stem cell derived cardiac fibroblasts
-
Article
Open AccessMechanical stimulation of induced pluripotent stem cell derived cardiac fibroblasts
Cardiac fibrosis contributes to the development of heart failure, and is the response of cardiac fibroblasts (CFs) to pressure or volume overload. Limiting factors in CFs research are the poor availability of ...
-
Article
Open AccessVitamin C facilitates direct cardiac reprogramming by inhibiting reactive oxygen species
After myocardial infarction, the lost myocardium is replaced by fibrotic tissue, eventually progressively leading to myocardial dysfunction. Direct reprogramming of fibroblasts into cardiomyocytes via the forc...
-
Article
Open AccessSMAD6-deficiency in human genetic disorders
SMAD6 encodes an intracellular inhibitor of the bone morphogenetic protein (BMP) signalling pathway. Until now, SMAD6-deficiency has been associated with three distinctive human congenital conditions, i.e., conge...
-
Article
Open AccessInhibition of the prolyl isomerase Pin1 improves endothelial function and attenuates vascular remodelling in pulmonary hypertension by inhibiting TGF-β signalling
Pulmonary arterial hypertension (PAH) is a devastating disease, characterized by obstructive pulmonary vascular remodelling ultimately leading to right ventricular (RV) failure and death. Disturbed transformin...
-
Article
Open AccessExacerbated inflammatory signaling underlies aberrant response to BMP9 in pulmonary arterial hypertension lung endothelial cells
Imbalanced transforming growth factor beta (TGFβ) and bone morphogenetic protein (BMP) signaling are postulated to favor a pathological pulmonary endothelial cell (EC) phenotype in pulmonary arterial hypertens...
-
Article
Open AccessCardiac Progenitor Cell–Derived Extracellular Vesicles Reduce Infarct Size and Associate with Increased Cardiovascular Cell Proliferation
Cell transplantation studies have shown that injection of progenitor cells can improve cardiac function after myocardial infarction (MI). Transplantation of human cardiac progenitor cells (hCPCs) results in an...
-
Protocol
Immunofluorescent Visualization of BMP Signaling Activation on Paraffin-Embedded Tissue Sections
Immunohistochemistry allows the detection of the presence, localization, and activation of proteins in biological tissues by using the ability of antibodies to bind to specific antigens. Cellular signaling can...
-
Article
Spheroid three-dimensional culture enhances Notch signaling in cardiac progenitor cells
Cardiac progenitor cells (CPCs) are a promising candidate for cardiac regeneration, and the interaction between CPCs and their microenvironment can influence their regenerative response. Notch signaling plays ...
-
Article
Open AccessTGFβ1-induced SMAD2/3 and SMAD1/5 phosphorylation are both ALK5-kinase-dependent in primary chondrocytes and mediated by TAK1 kinase activity
Dysregulated transforming growth factor β (TGFβ) signaling is implicated in osteoarthritis development, making normalizing TGFβ signaling a possible therapy. Theoretically, this can be achieved with small mole...
-
Article
Open AccessHuman fetal and adult epicardial-derived cells: a novel model to study their activation
The epicardium, a cell layer covering the heart, plays an important role during cardiogenesis providing cardiovascular cell types and instructive signals, but becomes quiescent during adulthood. Upon cardiac i...
-
Article
Open AccessHistopathology of aortic complications in bicuspid aortic valve versus Marfan syndrome: relevance for therapy?
Patients with bicuspid aortic valve (BAV) and patients with Marfan syndrome (MFS) are more prone to develop aortic dilation and dissection compared to persons with a tricuspid aortic valve (TAV). To elucidate ...
-
Article
Inhibition of TGFβ type I receptor activity facilitates liver regeneration upon acute CCl4 intoxication in mice
Liver exhibits a remarkable maintenance of functional homeostasis in the presence of a variety of damaging toxic factors. Tissue regeneration involves cell replenishment and extracellular matrix remodeling. K...
-
Article
Nuclear receptor NR4A1 promotes breast cancer invasion and metastasis by activating TGF-β signalling
In advanced cancers, the TGF-β pathway acts as an oncogenic factor and is considered to be a therapeutic target. Here using a genome-wide cDNA screen, we identify nuclear receptor NR4A1 as a strong activator o...
-
Article
microRNA-1 enhances the angiogenic differentiation of human cardiomyocyte progenitor cells
Instigated by the discovery of adult cardiac progenitor cells, cell replacement therapy has become a promising option for myocardial repair in the past decade. We have previously shown that human-derived cardi...
-
Chapter
TGF-β and Cardiovascular Disorders
Transforming growth factor β (TGF-β) is a secreted pleiotropic cytokine that is involved in a wide range of biological processes and has essential roles in development and tissue homeostasis. TGF-β elicits cel...
-
Protocol
Isolation and Differentiation of Human Cardiomyocyte Progenitor Cells into Cardiomyocytes
To date, there is no suitable in vitro model to study human adult cardiac cell biology. Here, we describe a method for efficient isolation and expansion of human cardiomyocyte progenitor cells (CMPCs) from car...
-
Article
Open AccessAge-dependent alteration of TGF-β signalling in osteoarthritis
Osteoarthritis (OA) is a disease of articular cartilage, with aging as the main risk factor. In OA, changes in chondrocytes lead to the autolytic destruction of cartilage. Transforming growth factor-β has rece...
-
Article
Open AccessIn vitro epithelial-to-mesenchymal transformation in human adult epicardial cells is regulated by TGFβ-signaling and WT1
Adult epicardial cells are required for endogenous cardiac repair. After myocardial injury, they are reactivated, undergo epithelial-to-mesenchymal transformation (EMT) and migrate into the injured myocardium ...
-
Article
Open AccessActive Wnt signaling in response to cardiac injury
Although the contribution of Wnt signaling in infarct healing is suggested, its exact role after myocardial infarction (MI) still needs to be unraveled. We evaluated the cardiac presence of active Wnt signalin...
