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Protocol
Assessment of HDAC Inhibitor-Induced Endoplasmic Reticulum (ER) Stress
The endoplasmic reticulum (ER) is a multifunctional cell organelle which is important for the folding and processing of proteins. Different endogenous and exogenous factors can disturb the ER homeostasis, caus...
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Protocol
Assessment of Mitochondrial Dysfunctions After Sirtuin Inhibition
Posttranslational modifications are important for protein functions and cellular signaling pathways. The acetylation of lysine residues is catalyzed by histone acetyltransferases (HATs) and removed by histone ...
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Article
Open AccessIn vitro-Amplifikation humaner hämatopoetischer Stammzellen im 3D-System
A promising strategy to increase the numbers of hematopoietic stem cells (HSCs) for clinical applications, like stem cell transplantation, is offered by advanced in vitro culture systems. We developed artificial ...
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Article
Open AccessGlobal metabolic alterations in colorectal cancer cells during irinotecan-induced DNA replication stress
Metabolic adaptations can allow cancer cells to survive DNA-damaging chemotherapy. This unmet clinical challenge is a potential vulnerability of cancer. Accordingly, there is an intense search for mechanisms t...
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Article
Open AccessTight association of autophagy and cell cycle in leukemia cells
Autophagy plays an essential role in maintaining cellular homeostasis and in the response to cellular stress. Autophagy is also involved in cell cycle progression, yet the relationship between these processes ...
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Article
Open AccessMolecular characterization of hematopoietic stem cells after in vitro amplification on biomimetic 3D PDMS cell culture scaffolds
Hematopoietic stem cell (HSC) transplantation is successfully applied since the late 1950s. However, its efficacy can be impaired by insufficient numbers of donor HSCs. A promising strategy to overcome this hu...
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Article
Open AccessCooperative treatment effectiveness of ATR and HSP90 inhibition in Ewing’s sarcoma cells
Ewing's sarcoma is an aggressive childhood malignancy whose outcome has not substantially improved over the last two decades. In this study, combination treatments of the HSP90 inhibitor AUY922 with either the...
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Article
The sirtuin 1/2 inhibitor tenovin-1 induces a nonlinear apoptosis-inducing factor-dependent cell death in a p53 null Ewing’s sarcoma cell line
The sirtuin 1/2 inhibitor tenovin-1 activates p53 and may have potential in the management of cancer. Here, we investigated the responsiveness of Ewing’s sarcoma cells to tenovin-1. We examined its effects in ...
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Protocol
Assessment of HDACi-Induced Cytotoxicity
The chromatin contains the genetic and the epigenetic information of a eukaryotic organism. Posttranslational modifications of histones, such as acetylation and methylation, regulate their structure and contro...
