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Article
Erratum: ERG deletion is associated with CD2 and attenuates the negative impact of IKZF1 deletion in childhood acute lymphoblastic leukemia
Correction to: Leukemia (2014) 28, 182–185; doi: 10.1038/leu.2013.282; published online 18 October 2013 There is an incorrect sequence for the primer ‘reverse 2’ in Supplementary Information (Methods, page 2, ...
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Article
ERG deletion is associated with CD2 and attenuates the negative impact of IKZF1 deletion in childhood acute lymphoblastic leukemia
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Article
Monitoring treatment response of childhood precursor B-cell acute lymphoblastic leukemia in the AIEOP-BFM-ALL 2000 protocol with multiparameter flow cytometry: predictive impact of early blast reduction on the remission status after induction
Treatment response is a strong outcome predictor for childhood acute lymphoblastic leukemia (ALL). Here, we evaluated the predictive impact of flow cytometric blast quantification assays (absolute blast count,...
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Article
Discriminant function analysis as decision support system for the diagnosis of acute leukemia with a minimal four color screening panel and multiparameter flow cytometry immunophenoty**
Despite several recommendations for standardization of multiparameter flow cytometry (MFC) the number, specificity and combinations of reagents used by diagnostic laboratories for the diagnosis and classificat...
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Article
Gene expression shift towards normal B cells, decreased proliferative capacity and distinct surface receptors characterize leukemic blasts persisting during induction therapy in childhood acute lymphoblastic leukemia
In childhood acute lymphoblastic leukemia (ALL), persistence of leukemic blasts during therapy is of crucial prognostic significance. In the present study, we address molecular and cell biologic features of bl...
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Article
Identification of deficient mitochondrial signaling in apoptosis resistant leukemia cells by flow cytometric analysis of intracellular cytochrome c, caspase-3 and apoptosis
Deficient activation of apoptosis signaling pathways may be responsible for treatment failure of malignant diseases. In primary leukemia samples the detection of deficient mitochondrial apoptosis signaling wou...
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Article
Efficient elimination of chronic lymphocytic leukaemia B cells by autologous T cells with a bispecific anti-CD19/anti-CD3 single-chain antibody construct
Recently, we have shown that a novel recombinant bispecific single-chain antibody construct (bscCD19 × CD3), induces highly efficacious lymphoma-directed cytotoxicity mediated by unstimulated peripheral T lymp...
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Article
Coexpression of p21WAF1/CIP1 in adenovirus vector transfected human primary hepatocytes prevents apoptosis resulting in improved transgene expression
Replication-deficient adenovirus (Ad vector) is one of the most effective gene transfer systems. However, its employment in human gene therapy trials is hampered by Ad vector associated cytotoxicity and induct...
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Chapter and Conference Paper
In Vitro Sensitivity to Chemotherapy-Induced Apoptosis in CD2+ve and CD2-ve Childhood T-All
T-lineage acute lymphoblastic leukemia (T- ALL) account for approximately 15% of all childhood acute leukemias and has been reported to have a worse prognosis than B- lineage ALL (Reiter et al., 1994; Uckun et...
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Article
In vitro susceptibility to TRAIL-induced apoptosis of acute leukemia cells in the context of TRAIL receptor gene expression and constitutive NF-κB activity
The TNF-related apoptosis-inducing ligand (TRAIL) is currently under evaluation as a possible (co-)therapeutic in cancer treatment. We therefore examined 129 cell samples from patients with de novo acute leukemia...
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Article
Apoptotic response to homoharringtonine in human wt p53 leukemic cells is independent of reactive oxygen species generation and implicates Bax translocation, mitochondrial cytochrome c release and caspase activation
In the present study, we investigated the in vitro apoptotic response of leukemic cells to the cellular stress induced by homoharringtonine (HHT), a plant alkaloid with antileukemic activity which is currently be...
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Chapter and Conference Paper
Involvement of BCL-2 and Bax in the IL-7-lnduced Inhibition of Spontaneous Apoptosis in Childhood T-ALL
Induction of apoptosis by cytokine deprivation and its inhibition by cytokines in growth factor-dependent cell lines has been demonstrated to be under the control of Bcl-2-related proteins. Recently, we have d...
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Article
Detection of acute leukemia cells with mixed lineage leukemia (MLL) gene rearrangements by flow cytometry using monoclonal antibody 7.1
Translocations involving 11q23 are among the most common genetic abnormalities in hematologic malignancies, occurring in approximately 5–10% of acute lymphoblastic leukemia (ALL) and 5% of acute myeloblastic l...
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Article
Clinical significance of CD95, Bcl-2 and Bax expression and CD95 function in adult de novo acute myeloid leukemia in context of P-glycoprotein function, maturation stage, and cytogenetics
Resistance to chemotherapy-induced apoptosis and a multidrug-resistance (MDR) phenotype, mainly mediated by P-glycoprotein (P-gp), contribute to chemotherapy failure in hematologic malignancies. To study apopt...
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Article
Immunophenotype and clinical characteristics of CD45-negative and CD45-positive childhood acute lymphoblastic leukemia
To evaluate the expression pattern of the leukocyte common antigen CD45 in acute leukemias and to investigate whether the lack of CD45 expression in childhood acute lymphoblastic leukemia (ALL) is associated ...
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Chapter and Conference Paper
CD95-Mediated Apoptosis in Acute Myeloid Leukemia (AML): Dependence on Maturational Stage and Growth Characteristics in Vitro
The CD95 (APO-1/Fas) receptor, which is able to induce apoptotic cell death upon oligomerization by its natural ligand or by anti-CD95 antibodies, is broadly distributed in normal and malignant hematopoietic c...
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Article
Differential CD95 expression and function in T and B lineage acute lymphoblastic leukemia cells
CD95 (Fas/APO-1) is a cell surface receptor able to trigger apoptosis in a variety of cell types. The expression and function of the CD95 antigen on leukemic blasts from 42 patients with B lineage and 53 patie...
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Chapter and Conference Paper
Untersuchungen zur Mastzellbiologie mittels monoklonaler Antikörper
Trotz einer steigenden Zahl von Arbeiten zu Fragen der Mastzelldifferenzierung ist der genaue Differenzierungsweg der Mastzellen noch immer unklar. Ziel dieser Arbeit war daher, durch Erzeugung monoklonaler An...
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Chapter and Conference Paper
Herstellung und Nutzung bispezifischer monoklonaler Antikörper
Die unter natürlichen Bedingungen produzierten Antikörper sind in ihrer Grundstruktur monospezifisch, d.h. alle Antigenbindungsorte des Antikörpermoleküls sind identisch. Die praktische Nutzung von Antikörpern...
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Article
Monoclonal antibodies against rat mast cells differentiate between subtypes
Four monoclonal mouse anti rat mast cell antibodies were selected which detect an antigenic determinant occuring on connective tissue mast cells of the rat. A strong antigen density was found on peritoneal mas...
