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Article
Open AccessChaperone mediated detection of small molecule target binding in cells
The ability to quantitatively measure a small molecule’s interactions with its protein target(s) is crucial for both mechanistic studies of signaling pathways and in drug discovery. However, current methods to...
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Article
The RIPK4–IRF6 signalling axis safeguards epidermal differentiation and barrier function
The integrity of the mammalian epidermis depends on a balance of proliferation and differentiation in the resident population of stem cells1. The kinase RIPK4 and the transcription factor IRF6 are mutated in seve...
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Article
Open AccessReactive-site-centric chemoproteomics identifies a distinct class of deubiquitinase enzymes
Activity-based probes (ABPs) are widely used to monitor the activity of enzyme families in biological systems. Inferring enzyme activity from probe reactivity requires that the probe reacts with the enzyme at ...
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Article
USP7 small-molecule inhibitors interfere with ubiquitin binding
The development of selective ubiquitin-specific protease-7 (USP7) inhibitors GNE-6640 and GNE-6776, which induce tumour cell death and reveal differential kinetics of Lys-48 and Lys-63-linked ubiquitin chain d...
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Article
Open AccessCoordinated ubiquitination and phosphorylation of RIP1 regulates necroptotic cell death
Proper regulation of cell death signaling is crucial for the maintenance of homeostasis and prevention of disease. A caspase-independent regulated form of cell death called necroptosis is rapidly emerging as a...
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Article
Erratum: Phosphorylation and linear ubiquitin direct A20 inhibition of inflammation
Nature 528, 370–375 (2015); doi: 10.1038/nature16165. In this Article, owing to a typesetter error the ‘received date’ was incorrectly shown as ‘5 November 2015’ instead of ‘5 November 2013’; this has been cor...
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Article
Phosphorylation and linear ubiquitin direct A20 inhibition of inflammation
Inactivation of the TNFAIP3 gene, encoding the A20 protein, is associated with critical inflammatory diseases including multiple sclerosis, rheumatoid arthritis and Crohn’s disease. However, the role of A20 in at...
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Article
Open AccessNovel Cul3 binding proteins function to remodel E3 ligase complexes
Cullins belong to a family of scaffold proteins that assemble multi-subunit ubiquitin ligase complexes to recruit protein substrates for ubiquitination via unique sets of substrate adaptor, such as Skp1 or Elo...
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Chapter and Conference Paper
Phosphoproteomic Analysis of Lymphocyte Signaling
Through fusion of innovations in high-throughput chromatographic separations of phosphopeptides, detection by mass spectrometry, and bioinformatic analysis, we have assembled a formidable tool to complement th...
