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Article
Evolutionary genomics of anthroponosis in Cryptosporidium
Human cryptosporidiosis is the leading protozoan cause of diarrhoeal mortality worldwide, and a preponderance of infections is caused by Cryptosporidium hominis and C. parvum. Both species consist of several subt...
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Article
Open AccessTerminator Operon Reporter: combining a transcription termination switch with reporter technology for improved gene synthesis and synthetic biology applications
Synthetic biology is characterized by the development of novel and powerful DNA fabrication methods and by the application of engineering principles to biology. The current study describes Terminator Operon Re...
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Article
Open AccessSeasonal immunoregulation in a naturally-occurring vertebrate
Fishes show seasonal patterns of immunity, but such phenomena are imperfectly understood in vertebrates generally, even in humans and mice. As these seasonal patterns may link to infectious disease risk and in...
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Article
Open AccessGeneration of whole genome sequences of new Cryptosporidium hominis and Cryptosporidium parvum isolates directly from stool samples
Whole genome sequencing (WGS) of Cryptosporidium spp. has previously relied on propagation of the parasite in animals to generate enough oocysts from which to extract DNA of sufficient quantity and purity for ana...
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Article
Open AccessRapGene: a fast and accurate strategy for synthetic gene assembly in Escherichia coli
The ability to assemble DNA sequences de novo through efficient and powerful DNA fabrication methods is one of the foundational technologies of synthetic biology. Gene synthesis, in particular, has been considere...
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Article
Sequence and analysis of chromosome 2 of Dictyostelium discoideum
The genome of the lower eukaryote Dictyostelium discoideum comprises six chromosomes. Here we report the sequence of the largest, chromosome 2, which at 8 megabases (Mb) represents about 25% of the genome. Despit...
