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Open AccessCorrection to: RNAseq and quantitative proteomic analysis of Dictyostelium knock-out cells lacking the core autophagy proteins ATG9 and/or ATG16
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Open AccessRNAseq and quantitative proteomic analysis of Dictyostelium knock-out cells lacking the core autophagy proteins ATG9 and/or ATG16
Autophagy is an evolutionary ancient mechanism that sequesters substrates for degradation within autolysosomes. The process is driven by many autophagy-related (ATG) proteins, including the core members ATG9 a...
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Dictyostelium Host Response to Legionella Infection: Strategies and Assays
The professional phagocyte Dictyostelium discoideum is a well-established model organism to study host-pathogen interactions. Dictyostelium amoebae grow as separate, independent cells; they divide by binary fissi...
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Open AccessThe function of the inner nuclear envelope protein SUN1 in mRNA export is regulated by phosphorylation
SUN1, a component of the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex, functions in mammalian mRNA export through the NXF1-dependent pathway. It associates with mRNP complexes by direct interaction...
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Open AccessA set of genes conserved in sequence and expression traces back the establishment of multicellularity in social amoebae
The developmental cycle of Dictyostelid amoebae represents an early form of multicellularity with cell type differentiation. Mutant studies in the model Dictyostelium discoideum revealed that its developmental pr...
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Open AccessCoronin7 regulates WASP and SCAR through CRIB mediated interaction with Rac proteins
Coronin7 (CRN7) stabilizes F-actin and is a regulator of processes associated with the actin cytoskeleton. Its loss leads to defects in phagocytosis, motility and development. It harbors a CRIB (Cdc42- and Rac...
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Open AccessCoronin 2A (CRN5) expression is associated with colorectal adenoma-adenocarcinoma sequence and oncogenic signalling
Coronin proteins are known as regulators of actin-based cellular processes, and some of them are associated with the malignant progression of human cancer. Here, we show that expression of coronin 2A is up-reg...
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Dictyostelium Host Response to Legionella Infection: Strategies and Assays
The professional phagocyte Dictyostelium discoideum is a simple eukaryotic microorganism, whose natural habitat is deciduous forest soil and decaying leaves, where the amoebae feed on bacteria and grow as separat...
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Open AccessPhosphorylation of CRN2 by CK2 regulates F-actin and Arp2/3 interaction and inhibits cell migration
CRN2 (synonyms: coronin 1C, coronin 3) functions in the re-organization of the actin network and is implicated in cellular processes like protrusion formation, secretion, migration and invasion. We demonstrate...
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Open AccessComparative genomics of the social amoebae Dictyostelium discoideum and Dictyostelium purpureum
The social amoebae (Dictyostelia) are a diverse group of Amoebozoa that achieve multicellularity by aggregation and undergo morphogenesis into fruiting bodies with terminally differentiated spores and stalk ce...
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Redundant and unique roles of coronin proteins in Dictyostelium
Dictyostelium discoideum harbors a short (CRN12) and a long coronin (CRN7) composed of one and two beta-propellers, respectively. They are primarily present in the cell cortex and cells lacking CRN12 (corA−) or C...
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Open AccessLarge scale multiplex PCR improves pathogen detection by DNA microarrays
Medium density DNA microchips that carry a collection of probes for a broad spectrum of pathogens, have the potential to be powerful tools for simultaneous species identification, detection of virulence factor...
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Chapter
The Coronin Family of Proteins
The coronins, first described in Dictyostelium discoideum in 1991, have meanwhile been detected in all eukaryotes except plants. They belong to the superfamily of WD40-repeat proteins and represent a large family...
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Evolutionary and Functional Diversity of Coronin Proteins
This chapter discusses various aspects of coronin phylogeny, structure and function that are of specific interest. Two subfamilies of ancient coronins of unicellular pathogens such as Entamoeba, Trypanosoma, Leis...
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Open AccessSTATc is a key regulator of the transcriptional response to hyperosmotic shock
Dictyostelium discoideum is frequently subjected to environmental changes in its natural habitat, the forest soil. In order to survive, the organism had to develop effective mechanisms to sense and respond to suc...
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Analysis of Gene Expression Using cDNA Microarrays
A DNA microarray consists of an orderly arrangement of DNA, cDNA, or oligonucleotide probes that represent individual genes of an organism. Today, microarrays are the most important tools used to analyze gene ...
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Open AccessThe Dictyostelium genome encodes numerous RasGEFs with multiple biological roles
Dictyostelium discoideum is a eukaryote with a simple lifestyle and a relatively small genome whose sequence has been fully determined. It is widely used for studies on cell signaling, movement and multicellular ...
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Revamp a model—status and prospects of the Dictyostelium genome project
International efforts are underway that aim at determining the complete genome sequence of the social amoeba Dictyostelium discoideum. As strategy, a whole chromosome shotgun (WCS) approach was chosen and each o...
