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Article
Open AccessImplementing turnaround strategies as an entrepreneurial process
Recovery strategy belongs to the group of rescue/reversing business strategies and is used mainly by companies facing financial or other problems which they are trying to overcome. Moreover, this strategy is a...
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Protocol
Optimized Aequorin Reconstitution Protocol to Visualize Calcium Ion Transients in the Heart of Transgenic Zebrafish Embryos In Vivo
We introduce how to image calcium ion levels in the heart of zebrafish embryos and larvae up to 5 days post-fertilization with the photoprotein green fluorescent protein (GFP)-aequorin (GA) in the transgenic l...
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Article
Open AccessImaging Ca2+ activity in mammalian cells and zebrafish with a novel red-emitting aequorin variant
Ca2+ monitoring with aequorin is an established bioluminescence technique, whereby the photoprotein emits blue light when it binds to Ca2+. However, aequorin’s blue emission and low quantum yield limit its applic...
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Article
Relationships with Citizens in Public Management: A Study at the Largest Spanish Town Halls
This paper provides an analysis of the relationships existing between citizen participation and satisfaction levels within the framework of Spanish local administrations, additionally paying attention to the l...
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Article
Information systems offshore outsourcing: managerial conclusions from academic research
In the light of the growing interest raised by Information Systems Offshore Outsourcing both in the managerial world and in the academic arena, the present work carries out a revision of the research in this a...
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Article
Discrimination between alternate membrane protein topologies in living cells using GFP/YFP tagging and pH exchange
Membrane protein function is determined by the relative organization of the protein domains with respect to the membrane. We have experimentally verified the topology of a protein with diverse orientations ari...
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Article
Red and green aequorins for simultaneous monitoring of Ca2+ signals from two different organelles
Simultaneous control of different functions by calcium signals is possible because of subcellular compartmentalization. Targeted chemiluminescent Ca2+ probes, such as aequorins (AEQs) are optimal for detecting si...
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Article
Corporate governance and organisational culture: The role of ethics officers
We argue that ethical leadership must be in line with corporate governance in general and of the Chief Executive Officer (CEO) who must be the starting point for the rest of the organisational members to have ...
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Article
Open AccessOxidative stress is increased in critically ill patients according to antioxidant vitamins intake, independent of severity: a cohort study
Critically ill patients suffer from oxidative stress caused by reactive oxygen species (ROS) and reactive nitrogen species (RNS). Although ROS/RNS are constantly produced under normal circumstances, critical i...
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Article
Factors influencing the intake and plasma levels of calcium, phosphorus and magnesium in southern Spain
The aim of the study was to assess factors influencing the intake and plasma levels of calcium (Ca), phosphorus (P) and magnesium (Mg) in an adult population from southern Spain, in order to identify patterns ...
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Article
A Corporate Culture Pattern to Manage Business Ethics
Within the general frame of proposals for an adequate management of business ethics, this paper is based on the vision of corporate culture as a pattern to achieve such purpose. If we consider ethics as a spec...
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Changes in intramitochondrial and cytosolic pH: early events that modulate caspase activation during apoptosis
Mitochondria trigger apoptosis by releasing caspase activators, including cytochrome c (cytC). Here we show, using a pH-sensitive green fluorescent protein (GFP), that mitochondria-dependent apoptotic stimuli (su...
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Article
Cell-permeant caged InsP3 ester shows that Ca2+ spike frequency can optimize gene expression
Inositol 1,4,5-trisphosphate (InsP3) releases calcium from intracellular stores and triggers complex waves and oscillations in levels of cytosolic free calcium1,2,3,4,5,. To determine which longer-term responses ...
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Article
Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin
Important Ca2+ signals in the cytosol and organelles are often extremely localized and hard to measure. To overcome this problem we have constructed new fluorescent indicators for Ca2+ that are genetically encode...
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Article
Cytosolic Ca2+ spikes evoked by the thiol reagent thimerosal in both intact and internally perfused single pancreatic acinar cells
Cytosolic calcium signals evoked by the sulphydryl-group-oxidising agent, thimerosal, have been investigated in acutely isolated pancreatic acinar cells. Two techniques were employed for the assessment of the ...
