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    Cloning, construction of prokaryotic expression vector and expression ofEscherichia coli cytosine deaminase gene

    Cytosine deaminase gene ofEscherichia coli strain H-30 was cloned, and its initiation codon of ‘GTG’ was mutated to ‘ATG’ by PCR. Prokaryotic recombinant expression vector pBV220-CD was constructed. Clone with hi...

    Shengjun Ren, Huiqiu Jiang, Jianren Gu in Chinese Science Bulletin (1998)