Abstract
Cytosine deaminase gene ofEscherichia coli strain H-30 was cloned, and its initiation codon of ‘GTG’ was mutated to ‘ATG’ by PCR. Prokaryotic recombinant expression vector pBV220-CD was constructed. Clone with high enzyme activity were selected by detecting their specific activity of cytosine deaminase. 5-FC(5-FC, 5-fluorocytosine) could induce the lethal toxicity to cells containing active CD gene. DNA sequence analysis indicated that there were 16 altered bases and 5 of them resulted in the alteration of amino acids in predicted peptide by comparing DNA sequence of the clone H-30-CD-11 with high enzyme activity with CD gene reported in Gene Bank.
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Ren, S., Jiang, H. & Gu, J. Cloning, construction of prokaryotic expression vector and expression ofEscherichia coli cytosine deaminase gene. Chin.Sci.Bull. 43, 223–230 (1998). https://doi.org/10.1007/BF02898917
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DOI: https://doi.org/10.1007/BF02898917