Diabetic Retinopathy
Methods and Protocols
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Open AccessAdeno-associated virus as a delivery vector for gene therapy of human diseases
Adeno-associated virus (AAV) has emerged as a pivotal delivery tool in clinical gene therapy owing to its minimal pathogenicity and ability to establish long-term gene expression in different tissues. Recombin...
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Protocol
Methods for Ex Vivo AAV Transduction in Rodent and Human Retinal Explants
Translational research is heavily dependent on animal models, and reliable disease models are essential for the development of novel therapies. Here, we outline the methods for culturing mouse and human retina...
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Protocol
Bioinformatics Tools for Bulk Gene Expression Deconvolution in Diabetic Retinopathy
Retinal neovascularization is one of the leading causes of vision loss and a hallmark of proliferative diabetic retinopathy (PDR). The immune system is observed to be involved in the pathogenesis of diabetic r...
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Open AccessAAV2-mediated gene therapy for Bietti crystalline dystrophy provides functional CYP4V2 in multiple relevant cell models
Bietti crystalline dystrophy (BCD) is an inherited retinal disease (IRD) caused by mutations in the CYP4V2 gene. It is a relatively common cause of IRD in east Asia. A number of features of this disease make it h...
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Open AccessComparative analysis of loop-mediated isothermal amplification (LAMP)-based assays for rapid detection of SARS-CoV-2 genes
The COVID-19 pandemic caused by SARS-CoV-2 has infected millions worldwide, therefore there is an urgent need to increase our diagnostic capacity to identify infected cases. Although RT-qPCR remains the gold s...
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TAK1 signaling is a potential therapeutic target for pathological angiogenesis
Angiogenesis plays a critical role in both physiological responses and disease pathogenesis. Excessive angiogenesis can promote neoplastic diseases and retinopathies, while inadequate angiogenesis can lead to ...
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A drug-tunable Flt23k gene therapy for controlled intervention in retinal neovascularization
Gene therapies that chronically suppress vascular endothelial growth factor (VEGF) represent a new approach for managing retinal vascular leakage and neovascularization. However, constitutive suppression of VE...
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Updates on Gene Therapy for Diabetic Retinopathy
Diabetic retinopathy (DR), a leading cause of visual impairment in the developed country, is characterized by vascular lesions and neuronal damage of the retina. Treatment options for this condition are curren...
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Protocol
Methods for Assessing Scaffold Vascularization In Vivo
The success of tissue engineering hinges on the rapid and sufficient vascularization of the neotissue. For efficient vascular network formation within three-dimensional (3D) constructs, biomaterial scaffolds t...
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Article
AAV-mediated gene delivery of the calreticulin anti-angiogenic domain inhibits ocular neovascularization
Ocular neovascularization is a common pathological feature in diabetic retinopathy and neovascular age-related macular degeneration that can lead to severe vision loss. We evaluated the therapeutic efficacy of...
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Protocol
Methods for In Vivo CRISPR/Cas Editing of the Adult Murine Retina
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein (Cas) is used by some bacteria and most archaea to protect against viral phage intrusion and has recently been adapt...
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Reply to: Effect of axial length on myopic choroidal neovascularization
