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Protocol
Retinal Assessment Using In Vivo Electroretinography and Optical Coherence Tomography in Rodent Models of Diabetes
Electroretinography and optical coherence tomography imaging allow for non-invasive quantitative assessment of the retina. These approaches have become mainstays for identifying the very earliest impact of hyp...
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Protocol
Measuring the Full-Field Electroretinogram in Rodents
Electroretinography allows for noninvasive functional assessment of the retina and is a mainstay for preclinical studies of retinal function in health and disease. The full-field electroretinogram is useful fo...
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Article
White matter tract conductivity is resistant to wide variations in paranodal structure and myelin thickness accompanying the loss of Tyro3: an experimental and simulated analysis
Myelination within the central nervous system (CNS) is crucial for the conduction of action potentials by neurons. Variation in compact myelin morphology and the structure of the paranode are hypothesised to h...
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Article
Open AccessCharacterization of retinal function and structure in the MPTP murine model of Parkinson’s disease
In addition to well characterized motor symptoms, visual disturbances are increasingly recognized as an early manifestation in Parkinson’s disease (PD). A better understanding of the mechanisms underlying thes...
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Article
Open AccessRetinal hyperspectral imaging in the 5xFAD mouse model of Alzheimer’s disease
Hyperspectral imaging of the retina has recently been posited as a potentially useful form of spectroscopy of amyloid-beta (Aβ) protein in the eyes of those with Alzheimer’s disease (AD). The concept of using ...
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Article
Open AccessRetinal ganglion cell dysfunction in mice following acute intraocular pressure is exacerbated by P2X7 receptor knockout
There is increasing evidence for the vulnerability of specific retinal ganglion cell (RGC) types in those with glaucoma and in animal models. In addition, the P2X7-receptor (P2X7-R) has been suggested to contr...
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Article
A drug-tunable Flt23k gene therapy for controlled intervention in retinal neovascularization
Gene therapies that chronically suppress vascular endothelial growth factor (VEGF) represent a new approach for managing retinal vascular leakage and neovascularization. However, constitutive suppression of VE...
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Article
Open AccessExperience-dependent development of visual sensitivity in larval zebrafish
The zebrafish (Danio rerio) is a popular vertebrate model for studying visual development, especially at the larval stage. For many vertebrates, post-natal visual experience is essential to fine-tune visual devel...
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Article
Open AccessNon-invasive in vivo hyperspectral imaging of the retina for potential biomarker use in Alzheimer’s disease
Studies of rodent models of Alzheimer’s disease (AD) and of human tissues suggest that the retinal changes that occur in AD, including the accumulation of amyloid beta (Aβ), may serve as surrogate markers of b...
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Chapter
Response of the Rat Optic Nerve to Acute Intraocular and Intracranial Pressure Changes
Glaucoma is a neurodegenerative disease, characterized by the progressive death of retinal ganglion cells. Elevated intraocular pressure (IOP) is known to be an important risk factor for glaucoma; however, it ...
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Article
Open AccessSystemic hypertension is not protective against chronic intraocular pressure elevation in a rodent model
High intraocular pressure is the most well documented glaucoma risk factor; however many patients develop and/or show progression of glaucoma in its absence. It is now thought that in some instances, ocular pe...
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Article
Open AccessAge-related changes in the response of retinal structure, function and blood flow to pressure modification in rats
Age-related changes to the balance between the pressure inside the eye (intraocular pressure, IOP) and the pressure inside the brain (intracranial pressure, ICP) can modify the risk of glaucoma. In this study,...
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Article
AAV-mediated gene delivery of the calreticulin anti-angiogenic domain inhibits ocular neovascularization
Ocular neovascularization is a common pathological feature in diabetic retinopathy and neovascular age-related macular degeneration that can lead to severe vision loss. We evaluated the therapeutic efficacy of...
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Chapter and Conference Paper
An Efficient Method for Mining Clickstream Patterns
Recently, hybrid approaches, which combine an FP-tree-like data structure with an interaction-based approach, are efficient approaches for mining frequent itemsets. However, applying those approaches for seque...
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Protocol
Methods for In Vivo CRISPR/Cas Editing of the Adult Murine Retina
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein (Cas) is used by some bacteria and most archaea to protect against viral phage intrusion and has recently been adapt...
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Article
Open AccessReactivity in the human retinal microvasculature measured during acute gas breathing provocations
Although changes in vessel diameter following gas perturbation have been documented in retinal arterioles and venules, these responses have yet to be quantified in the smallest vessels of the human retina. Her...
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Article
Electroretinography in streptozotocin diabetic rats following acute intraocular pressure elevation
We consider whether pre-existing streptozotocin induced hyperglycemia in rats affects the ability of the eye to cope with a single episode of acute intraocular pressure (IOP) elevation.
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Article
Glial and neuronal dysfunction in streptozotocin-induced diabetic rats
Neuronal dysfunction has been noted very soon after the induction of diabetes by streptozotocin injection in rats. It is not clear from anatomical evidence whether glial cell dysfunction accompanies the well-d...
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Article
Post-receptoral contributions to the rat scotopic electroretinogram a-wave
The electroretinogram is a widely used objective measure of visual function. The best characterised feature of the full-field dark-adapted flash ERG, is the earliest corneal negativity, the a-wave, which prima...
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Article
Dimethyl sulphoxide dose–response on rat retinal function
The aim of the study is to determine whether dimethyl sulphoxide (DMSO), a common laboratory solvent, impacts retinal function. Long Evans rats (n = 17) were intravitreally injected with five different doses of D...
