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  1. Article

    Open Access

    Improving the temperature characteristics and catalytic efficiency of a mesophilic xylanase from Aspergillus oryzae, AoXyn11A, by iterative mutagenesis based on in silico design

    To improve the temperature characteristics and catalytic efficiency of a glycoside hydrolase family (GHF) 11 xylanase from Aspergillus oryzae (AoXyn11A), its variants were predicted based on in silico design. Fir...

    Xue-Qing Li, Qin Wu, Die Hu, Rui Wang, Yan Liu, Min-Chen Wu, Jian-Fang Li in AMB Express (2017)

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  2. No Access

    Article

    Replacing a piece of loop-structure in the substrate-binding groove of Aspergillus usamii β-mannanase, AuMan5A, to improve its enzymatic properties by rational design

    To perfect the enzymatic properties of AuMan5A, a mesophilic glycoside hydrolase (GH) family 5 β-mannanase from Aspergillus usamii, its loop-structure substitution was carried out by rational design and followed ...

    Yun Hai Dong, Jian Fang Li, Die Hu, **n Yin… in Applied Microbiology and Biotechnology (2016)

  3. No Access

    Article

    Reduction of m-chlorophenacyl chloride coupled with regeneration of NADPH by recombinant Escherichia coli cells co-expressing both carbonyl reductase and glucose 1-dehydrogenase

    Both Sys1 and Sygdh, two codon-optimized genes encoding SyS1 and SyGDH, were synthesized based on the carbonyl reductase (S1) and glucose 1-dehydrogenase (GDH) gene sequences, respectively, from Candida magnoliae

    Tao Yu, Jian-Fang Li, Li-Juan Zhu, Die Hu, Chao Deng, Yu-Ting Cai… in Annals of Microbiology (2016)

  4. No Access

    Article

    Improvement in the thermostability of a type A feruloyl esterase, AuFaeA, from Aspergillus usamii by iterative saturation mutagenesis

    Feruloyl or ferulic acid esterase (Fae, EC 3.1.1.73) catalyzes the hydrolysis of ester bonds between polysaccharides and phenolic acid compounds in xylan side chain. In this study, the thermostability of a typ...

    **n Yin, Jian-Fang Li, Chun-Juan Wang, Die Hu… in Applied Microbiology and Biotechnology (2015)

  5. No Access

    Article

    Enhancing expression level of an acidophilic β-mannanase in Pichia pastoris by double vector system

    An acidophilic β-mannanase-encoding gene (Auman5A) from Aspergillus usamii YL-01-78 was amplified and inserted into pPIC9K and pPICZαA vectors. The resulting recombinant vector, pPIC9K-Auman5A, was transformed in...

    Cun-Duo Tang, **g Guo, Jian-Fang Li, **-Huan Wei, Die Hu… in Annals of Microbiology (2014)

  6. No Access

    Article

    Modified pPIC9K vector-mediated expression of a family 11 xylanase gene, Aoxyn11A, from Aspergillus oryzae in Pichia pastoris

    A full-length cDNA sequence of Aoxyn11A, a mesophilic xylanase-encoding gene from Aspergillus oryzae, was obtained from total RNA, using 3′ and 5′ rapid amplification of cDNA ends methods. The cDNA sequence is 1,...

    Jian-Fang Li, Shu-Juan Gao, **ao-Tong Liu, Yan-Yan Gong… in Annals of Microbiology (2013)

  7. No Access

    Article

    Determination of amino acids and dipeptides is correlated significantly with optimum temperatures of microbial lipases

    Amino acids and dipeptides that are correlated significantly with lipase optimum temperatures were searched for in 34 microbial lipase sequences by a stepwise regression method. The positive dipeptides were fo...

    Hui-Min Zhang, Jian-Fang Li, Min-Chen Wu, Hong-Ling Shi… in Annals of Microbiology (2013)