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Article
Failure mechanism of coal seam floor with concealed structure and evolution law of confined water seepage
Confined water inrush disasters occur frequently in Hanxing mining area in China. In order to deal with mine water inrush disasters effectively, it is necessary to master the point and speed of water inrush fr...
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Article
Open AccessPhosphodiesterase 4D polymorphisms associate with the short-term outcome in ischemic stroke
It has been demonstrated that phosphodiesterase 4D (PDE4D) genetic polymorphism is associated with ischemic stroke. However, the association between PDE4D gene and prognosis after ischemic stroke remains unkno...
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Article
Replacing a piece of loop-structure in the substrate-binding groove of Aspergillus usamii β-mannanase, AuMan5A, to improve its enzymatic properties by rational design
To perfect the enzymatic properties of AuMan5A, a mesophilic glycoside hydrolase (GH) family 5 β-mannanase from Aspergillus usamii, its loop-structure substitution was carried out by rational design and followed ...
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Article
Improvement in the thermostability of a type A feruloyl esterase, AuFaeA, from Aspergillus usamii by iterative saturation mutagenesis
Feruloyl or ferulic acid esterase (Fae, EC 3.1.1.73) catalyzes the hydrolysis of ester bonds between polysaccharides and phenolic acid compounds in xylan side chain. In this study, the thermostability of a typ...