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Article
Construction of Synthetic Microbial Consortium for Violacein Production
Violacein, a purple-colored natural pigment produced by microbes, has anti-microbial, anti-parasitic, and anti-cancer properties. Violacein can be synthesized through a complex pathway from a carbon source via...
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Article
Open AccessKinetic compartmentalization by unnatural reaction for itaconate production
Physical compartmentalization of metabolism using membranous organelles in eukaryotes is helpful for chemical biosynthesis to ensure the availability of substrates from competitive metabolic reactions. Bacteri...
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Article
Genetic Biocontainment Systems for the Safe Use of Engineered Microorganisms
Recent advances in genetic engineering and synthetic biology have enabled a variety of genetically modified microorganisms (GMOs) designed for open environmental applications. The use of GMOs in these applicat...
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Article
Sensitive fluorescence detection of SARS-CoV-2 RNA in clinical samples via one-pot isothermal ligation and transcription
The control of viral outbreaks requires nucleic acid diagnostic tests that are sensitive, simple and fast. Here, we report a highly sensitive and specific one-pot assay for the fluorescence-based detection of ...
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Article
Cell-free biosensors for rapid detection of water contaminants
Lack of access to safe drinking water is a global problem, and methods to reliably and easily detect contaminants could be transformative. We report the development of a cell-free in vitro transcription system...
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Article
Acetyl-CoA-derived biofuel and biochemical production in cyanobacteria: a mini review
Cyanobacteria, as photosynthetic bacteria, can generate energy and carbon sources directly from sunlight and atmospheric carbon dioxide. With the aid of genetic engineering, heterologous enzymes and/or pathway...
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Article
Next-generation biocontainment systems for engineered organisms
The increasing use of engineered organisms for industrial, clinical, and environmental applications poses a growing risk of spreading hazardous biological entities into the environment. To address this biosafe...
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Article
Comparison of end-tidal CO2 measured by transportable capnometer (EMMA™ capnograph) and arterial pCO2 in general anesthesia
An end-tidal CO2 monitor (capnometer) is used most often as a noninvasive substitute for PaCO2 in anesthesia, anesthetic recovery, and intensive care. Additionally, the wide spread on-site use of portable capnome...
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Article
'Deadman' and 'Passcode' microbial kill switches for bacterial containment
Synthetic biology has expanded the availability of engineered bacterial systems for diverse applications and is now develo** safeguards for their effective and secure use. The report of two synthetic gene ci...
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Article
Systems metabolic engineering of microorganisms for natural and non-natural chemicals
Growing concerns over limited fossil resources and associated environmental problems are motivating the development of sustainable processes for the production of chemicals, fuels and materials from renewable ...
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Chapter
Systems Metabolic Engineering of Escherichia coli for Chemicals, Materials, Biofuels, and Pharmaceuticals
Microorganisms are small but efficient micro-factories for producing bulk and value-added products. Systems biology and synthetic biology advanced during the last decade are enabling us to unravel the underlyi...
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Article
Development of sucrose-utilizing Escherichia coli K-12 strain by cloning β-fructofuranosidases and its application for l-threonine production
Sucrose is one of the most promising carbon sources for industrial fermentation. To achieve sucrose catabolism, the sucrose utilization operons have been introduced into microorganisms that are not able to uti...
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Article
Proteome-based physiological analysis of the metabolically engineered succinic acid producer Mannheimia succiniciproducens LPK7
Using two-dimensional gel electrophoresis (2-DE) and mass spectrometry, the proteome of a metabolically engineered succinic acid-overproducing bacterium, Mannheimia succiniciproducens LPK7, was examined and compa...
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Article
Simulation of atmospheric states for a storm surge on the west coast of Korea: model comparison between MM5, WRF and COAMPS
High-quality informations on sea level pressure and sea surface wind stress are required to accurately predict storm surges over the Korean Peninsula. The storm surge on 31 March 2007 at Yeonggwang, on the wes...
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Article
From genome sequence to integrated bioprocess for succinic acid production by Mannheimia succiniciproducens
Mannheimia succiniciproducens is a capnophilic gram-negative bacterium isolated from bovine rumen. Wild-type M. succiniciproducens can produce succinic acid as a major fermentation product with acetic, formic, an...
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Article
Growth and Characterization of InGaN/GaN LEDs on Corrugated Interface Substrate Using MOCVD
III-nitride films were grown on the corrugated interface substrate using a metalorganic chemical vapor deposition system to increase the optical power of white LEDs. The patterning of a substrate for enhancing...
